Identification of IncA Plasmid, Harboring <i>bla</i><sub>VIM-1</sub> Gene, in <i>S. enterica</i> Goldcoast ST358 and <i>C. freundii</i> ST62 Isolated in a Hospitalized Patient
Alessandra Piccirilli,
Sascia Di Marcantonio,
Venera Costantino,
Omar Simonetti,
Marina Busetti,
Roberto Luzzati,
Luigi Principe,
Marco Di Domenico,
Antonio Rinaldi,
Cesare Cammà,
Mariagrazia Perilli
Affiliations
Alessandra Piccirilli
Department of Biotechnological and Applied Clinical Sciences, University of L’Aquila, 67100 L’Aquila, Italy
Sascia Di Marcantonio
Department of Biotechnological and Applied Clinical Sciences, University of L’Aquila, 67100 L’Aquila, Italy
Venera Costantino
Microbiology Unit, Trieste University Hospital (ASUGI), 34125 Trieste, Italy
Omar Simonetti
Infectious Diseases Unit, Trieste University Hospital (ASUGI), 34125 Trieste, Italy
Marina Busetti
Microbiology Unit, Trieste University Hospital (ASUGI), 34125 Trieste, Italy
Roberto Luzzati
Infectious Diseases Unit, Trieste University Hospital (ASUGI), 34125 Trieste, Italy
Luigi Principe
Clinical Pathology and Microbiology Unit, “S. Giovanni di Dio” Hospital, 88900 Crotone, Italy
Marco Di Domenico
Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise, Campo Boario, 64100 Teramo, Italy
Antonio Rinaldi
Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise, Campo Boario, 64100 Teramo, Italy
Cesare Cammà
Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise, Campo Boario, 64100 Teramo, Italy
Mariagrazia Perilli
Department of Biotechnological and Applied Clinical Sciences, University of L’Aquila, 67100 L’Aquila, Italy
In the present study, we analyzed the genome of two S. enterica strains TS1 and TS2 from stool and blood cultures, respectively, and one strain of C. freundii TS3, isolated from a single hospitalized patient with acute myeloid leukemia. The S. enterica Goldcoast ST358 (O:8 (C2-C3) serogroup), sequenced by the MiSeq Illumina system, showed the presence of β-lactamase genes (blaVIM-1, blaSHV-12 and blaOXA-10), aadA1, ant(2″)-Ia, aac(6′)-Iaa, aac(6′)-Ib3, aac(6′)-Ib-cr, qnrVC6, parC(T57S), and several incompatibility plasmids. A wide variety of insertion sequences (ISs) and transposon elements were identified. In C. freundii TS3, these were the blaVIM-1, blaCMY-150, and blaSHV-12, aadA1, aac(6′)-Ib3, aac(6′)-Ib-cr, mph(A), sul1, dfrA14, ARR-2, qnrVC6, and qnrB38. IncA plasmid isolated from E.coli/K12 transconjugant and C. freundii exhibited a sequence identity >99.9%. The transfer of IncA plasmid was evaluated by conjugation experiments.
