Condensin Relocalization from Centromeres to Chromosome Arms Promotes Top2 Recruitment during Anaphase
Joanne Leonard,
Nicholas Sen,
Raul Torres,
Takashi Sutani,
Adam Jarmuz,
Katsuhiko Shirahige,
Luis Aragón
Affiliations
Joanne Leonard
Cell Cycle Group, Medical Research Council (MRC), Clinical Sciences Centre, Imperial College London, Hammersmith Hospital, Du Cane Road, London W12 0NN, UK
Nicholas Sen
Cell Cycle Group, Medical Research Council (MRC), Clinical Sciences Centre, Imperial College London, Hammersmith Hospital, Du Cane Road, London W12 0NN, UK
Raul Torres
Cell Cycle Group, Medical Research Council (MRC), Clinical Sciences Centre, Imperial College London, Hammersmith Hospital, Du Cane Road, London W12 0NN, UK
Takashi Sutani
Research Center for Epigenetic Disease, Institute of Molecular and Cellular Biosciences, The University of Tokyo, Tokyo 113-0032, Japan
Adam Jarmuz
Cell Cycle Group, Medical Research Council (MRC), Clinical Sciences Centre, Imperial College London, Hammersmith Hospital, Du Cane Road, London W12 0NN, UK
Katsuhiko Shirahige
Research Center for Epigenetic Disease, Institute of Molecular and Cellular Biosciences, The University of Tokyo, Tokyo 113-0032, Japan
Luis Aragón
Cell Cycle Group, Medical Research Council (MRC), Clinical Sciences Centre, Imperial College London, Hammersmith Hospital, Du Cane Road, London W12 0NN, UK
Condensin is a conserved chromosomal complex necessary to promote mitotic chromosome condensation and sister chromatid resolution during anaphase. Here, we report that yeast condensin binds to replicated centromere regions. We show that centromeric condensin relocalizes to chromosome arms as cells undergo anaphase segregation. We find that condensin relocalization is initiated immediately after the bipolar attachment of sister kinetochores to spindles and requires Polo kinase activity. Moreover, condensin localization during anaphase involves a higher binding rate on DNA and temporally overlaps with condensin’s DNA overwinding activity. Finally, we demonstrate that topoisomerase 2 (Top2) is also recruited to chromosome arms during anaphase in a condensin-dependent manner. Our results uncover a functional relation between condensin and Top2 during anaphase to mediate chromosome segregation.
