Communications Biology (Jun 2022)
TAS-Seq is a robust and sensitive amplification method for bead-based scRNA-seq
- Shigeyuki Shichino,
- Satoshi Ueha,
- Shinichi Hashimoto,
- Tatsuro Ogawa,
- Hiroyasu Aoki,
- Bin Wu,
- Chang-Yu Chen,
- Masahiro Kitabatake,
- Noriko Ouji-Sageshima,
- Noriyoshi Sawabata,
- Takeshi Kawaguchi,
- Toshitugu Okayama,
- Eiji Sugihara,
- Shigeto Hontsu,
- Toshihiro Ito,
- Yasunori Iwata,
- Takashi Wada,
- Kazuho Ikeo,
- Taka-Aki Sato,
- Kouji Matsushima
Affiliations
- Shigeyuki Shichino
- Division of Molecular Regulation of Inflammatory and Immune Diseases, Research Institute of Biomedical Sciences, Tokyo University of Science
- Satoshi Ueha
- Division of Molecular Regulation of Inflammatory and Immune Diseases, Research Institute of Biomedical Sciences, Tokyo University of Science
- Shinichi Hashimoto
- Department of Molecular Pathophysiology, Institute of Advanced Medicine, Wakayama Medical University
- Tatsuro Ogawa
- Division of Molecular Regulation of Inflammatory and Immune Diseases, Research Institute of Biomedical Sciences, Tokyo University of Science
- Hiroyasu Aoki
- Division of Molecular Regulation of Inflammatory and Immune Diseases, Research Institute of Biomedical Sciences, Tokyo University of Science
- Bin Wu
- Division of Molecular Regulation of Inflammatory and Immune Diseases, Research Institute of Biomedical Sciences, Tokyo University of Science
- Chang-Yu Chen
- Division of Molecular Regulation of Inflammatory and Immune Diseases, Research Institute of Biomedical Sciences, Tokyo University of Science
- Masahiro Kitabatake
- Department of Immunology, Nara Medical University
- Noriko Ouji-Sageshima
- Department of Immunology, Nara Medical University
- Noriyoshi Sawabata
- Department of Thoracic and Cardio-Vascular Surgery, Nara Medical University
- Takeshi Kawaguchi
- Department of Thoracic and Cardio-Vascular Surgery, Nara Medical University
- Toshitugu Okayama
- National Institute of Genetics
- Eiji Sugihara
- Research and Development Center for Precision Medicine, University of Tsukuba
- Shigeto Hontsu
- Department of Respiratory Medicine, Nara Medical University
- Toshihiro Ito
- Department of Immunology, Nara Medical University
- Yasunori Iwata
- Division of Infection Control, Kanazawa University Hospital, Department of Nephrology and Laboratory Medicine, Kanazawa University
- Takashi Wada
- Division of Infection Control, Kanazawa University Hospital, Department of Nephrology and Laboratory Medicine, Kanazawa University
- Kazuho Ikeo
- National Institute of Genetics
- Taka-Aki Sato
- Research and Development Center for Precision Medicine, University of Tsukuba
- Kouji Matsushima
- Division of Molecular Regulation of Inflammatory and Immune Diseases, Research Institute of Biomedical Sciences, Tokyo University of Science
- DOI
- https://doi.org/10.1038/s42003-022-03536-0
- Journal volume & issue
-
Vol. 5,
no. 1
pp. 1 – 15
Abstract
Terminator-assisted solid-phase cDNA amplification and sequencing (TASseq) uses a terminator, terminal transferase and bead-based platform to improve generation of single-cell RNA-seq libraries.
