Journal of the Serbian Chemical Society (Jan 2023)

Synthesis and antiproliferative activity of (5R)-cleistenolide and analogues

  • Farkas Sándor,
  • Benedeković Goran,
  • Stanisavljević Slađana M.,
  • Srećo-Zelenović Bojana M.,
  • Popsavin Mirjana,
  • Popsavin Velimir,
  • Jakimov Dimitar S.

DOI
https://doi.org/10.2298/JSC230126018F
Journal volume & issue
Vol. 88, no. 7-8
pp. 705 – 713

Abstract

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(5R)-Cleistenolide and a few related analogues have been synthesized starting from d-glucose. The key steps of the synthesis included a Z-selective Wittig olefination and an intramolecular Mitsunobu reaction with an inversion of configuration at the C-5 position. In vitro antiproliferative activity of synthesized compounds was tested on a panel of eight human tumour cells and against a single normal cell line (MRC-5). The majority of tested compounds showed strong antiproliferative effects on certain human tumour cells and all of them showed negligible toxicity to normal foetal lung fibroblasts (MRC-5). The most active compound obtained in this work is lactone 5, which in MDA-MB 231 cell culture showed the same activity as doxorubicin (IC50 0.09 μM). Strong antiproliferative activities of analogues 2, 5 and 6 were recorded in the K562 cell line (IC50 0.21, 0.34 and 0.33 μM, respectively), in which they showed very similar activities to doxorubicin (IC50 0.25 μM). A performed SAR study revealed that a change in the stereochemistry at the C-5 position may increase the activity of resulting stereoisomers.

Keywords