Fabrication of Nano Poly Cresol Red over Glassy Carbon Electrode and its Application in Selective Determination of Uric acid in the Presence of Ascorbic Acid

Abstract

A selective electrochemical method for the determination of uric acid was developed by using nano poly cresol red modified glassy carbon electrode. This new material has been characterized by Scanning Electron Microscopy, cyclic voltammetry and Differential pulse voltammetry. This modified electrode shows excellent electrocatalytic activity towards the oxidation of uric acid in the presence of ascorbic acid in (pH 7.0). Compare to bare electrode the modified electrode able to resolve the both ascorbic acid and uric acid in the solution mixture. The oxidation current of uric acid with the modified electrode is two folds higher that the bare glassy carbon electrode. Using differential pulse voltammetry method, the oxidation current is linear with the uric acid concentration in the range 30 µM - 575 µM with the detection limit of 5 µM (S/N = 3). Moreover uric acid in real samples can be determined using nano poly cresol red without any pretreatment of samples giving satisfactory results.

Keywords

• ascorbic acid
• electrocatalytic activity
• electron microscopy
• nano poly cresol red
• uric acid