Molecular Systems Biology (Nov 2008)

Hepatitis C virus infection protein network

  • B de Chassey,
  • V Navratil,
  • L Tafforeau,
  • M S Hiet,
  • A Aublin‐Gex,
  • S Agaugué,
  • G Meiffren,
  • F Pradezynski,
  • B F Faria,
  • T Chantier,
  • M Le Breton,
  • J Pellet,
  • N Davoust,
  • P E Mangeot,
  • A Chaboud,
  • F Penin,
  • Y Jacob,
  • P O Vidalain,
  • M Vidal,
  • P André,
  • C Rabourdin‐Combe,
  • V Lotteau

DOI
https://doi.org/10.1038/msb.2008.66
Journal volume & issue
Vol. 4, no. 1
pp. 1 – 12

Abstract

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Abstract A proteome‐wide mapping of interactions between hepatitis C virus (HCV) and human proteins was performed to provide a comprehensive view of the cellular infection. A total of 314 protein–protein interactions between HCV and human proteins was identified by yeast two‐hybrid and 170 by literature mining. Integration of this data set into a reconstructed human interactome showed that cellular proteins interacting with HCV are enriched in highly central and interconnected proteins. A global analysis on the basis of functional annotation highlighted the enrichment of cellular pathways targeted by HCV. A network of proteins associated with frequent clinical disorders of chronically infected patients was constructed by connecting the insulin, Jak/STAT and TGFβ pathways with cellular proteins targeted by HCV. CORE protein appeared as a major perturbator of this network. Focal adhesion was identified as a new function affected by HCV, mainly by NS3 and NS5A proteins.

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