Effect of phytase on phosphorous balance in 20-kg barrows fed low or adequate phosphorous diets

Abstract

The effects of phytase on phosphorus (P) digestibility are well established. However, there are few studies that report P balance, particularly when phytase is used in diets that have adequate or deficient P. The main objective of the study was to determine the effect of dietary P levels and exogenous phytase on P balance in growing pigs. The first part of the experiment was a 14-d metabolism study conducted with 80 barrows (initial body weight 18.5 ± 0.5 kg) with a 2 × 5 factorial arrangement of treatments and main effects of available P (0.13% available P, low P [Low-P] diet; 0.35% available P, adequate P [Adeq-P] diet) and phytase (0, 250, 500, 2,500, and 12,500 U/kg). A portion of the pigs (n = 24) fed the Low-P diet, with 0, 500, 2,500, 12,500 U/kg phytase, and those fed the Adeq-P diet, with 0 and 12,500 U/kg phytase, remained on test diets for another 4 d, and tissues were collected for determination of bone characteristics and tissue P concentration. There was a P × phytase interaction for P retention that was accounted for by a lack of response to phytase in pigs fed the Adeq-P diet. Retention of P was greater with incremental levels of phytase in pigs fed Low-P diets as compared to those fed Adeq-P diets (P level × phytase, P < 0.01), but calcium (Ca) retention was greater in pigs fed Adeq-P diets (P level × phytase P < 0.01). Apparent total tract digestibility (ATTD) of P was improved by phytase (P < 0.001) and was greater in pigs fed Adeq-P diets as compared to those fed Low-P diets (P = 0.006). Metatarsal bone ash (quadratic, P = 0.01) and strength (linear, P = 0.03) was increased by phytase addition to the Low-P diets. There were no phytase or dietary P effects on P concentrations of the heart, kidney, liver, muscle, and spleen. These results suggest that as compared to the effects in an Adeq-P diet, adding phytase to a Low-P diet was more effective at reducing the P and Ca excretion and restoring average daily gain (ADG). The P released by phytase is absorbed and contributes to improved bone growth, greater rates of tissue accretion, and increased body weight, but does not change tissue P concentrations. There is, however, a threshold for P retention, beyond which it is excreted in the urine. Keywords: Phosphorous, Phytase, Bone, Calcium, Digestibility, Tissue