Identification of Major Locus Bph35 Resistance to Brown Planthopper in Rice
Zhang Yuexiong,
Qin Gang,
Ma Qianqian,
Wei Minyi,
Yang Xinghai,
Ma Zengfeng,
Liang Haifu,
Liu Chi,
Li Zhenjing,
Liu Fang,
Huang Dahui,
Li Rongbai
Affiliations
Zhang Yuexiong
College of Agriculture and State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, Nanning 530007, China; Rice Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China
Qin Gang
Rice Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China
Ma Qianqian
College of Agriculture and State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, Nanning 530007, China
Wei Minyi
Rice Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China
Yang Xinghai
Rice Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China
Ma Zengfeng
Rice Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China
Liang Haifu
Rice Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China
Liu Chi
Rice Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China
Li Zhenjing
Rice Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China
Liu Fang
College of Agriculture and State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, Nanning 530007, China
Huang Dahui
Rice Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China; Corresponding authors.
Li Rongbai
College of Agriculture and State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, Nanning 530007, China; Corresponding authors.
An introgression line RBPH660, derived from wild rice Oryza rufipogon, showed stable resistance to brown planthopper (BPH). Segregation analysis indicated BPH resistance of RBPH660 was controlled by multiple genes/QTLs. By using the bulked segregant analysis (BSA)-seq method, two genomic regions harboring QTLs resistance to BPH were identified from 1.20 to 16.70 Mb on chromosome 4 and from 10.20 to 12.60 Mb on chromosome 9 in RBPH660, respectively. A major resistance locus, designated as Bph35 accounting for 51.27% of the phenotypic variation with a LOD score of 42.51, was mapped to the candidate region of chromosome 4 between InDel (insertion-deletion) markers PSM16 and R4M13. For fine mapping of Bph35, one simple sequence repeat and three newly developed InDel markers were used to screen the recombinants. Finally, the Bph35 locus was delimited in the region from 6.28 to 6.93 Mb and there were 18 predicted protein-encoding genes with a total of 114 non-synonymous single nucleotide polymorphism (SNP) variant sites between the resistant and susceptible parents. Out of these genes, Os04g0193950, encoding a putative NB-ARC (nucleotide- binding adaptor shared by APAF-1, R proteins and CED-4) and LRR (leucine-rich repeat) domain protein with nine non-synonymous SNP substitutions in its coding sequence regions, might be the candidate gene for Bph35. These findings would facilitate the map-based cloning of the Bph35 gene and development of resistant varieties against BPH in rice. Keywords: rice, brown planthopper, Bph35, bulked segregant analysis (BSA)-seq method, gene mapping
