Armaghane Danesh Bimonthly Journal (Apr 2018)
The Effect of Abelmoscus Esculentus Powder on Histological Changes of the Ovary in Diabetic Rats
Abstract
Background & Aim: Diabetes mellitus is the most common endocrine disorder in the world which causes ovarian dysfunction. The aim of this study was to evaluate the effect of omelette powder on ovarian tissue changes in diabetic rats using a high-fat diet based on streptozotocin HFD / STZ. Methods: In this experimental study, 25 female rats (Wistar rats) were divided into 5 groups of 5. The first group, as the control group, the second group was received high-fat diet (HFD) (fat 60% + 25% carbohydrate + protein 15%), followed by STZ (35 mg / kg) intravenous 4 weeks. the third group received diazepam (200 mg / kg), the fourth group received metformin (200 mg / kg), the fifth group as sham or the control group of healthy rats (200 mg / kg) received only Abelmoschus esculentus powder. After 4 weeks of treatment the animals were euthanized body weight, ovary weight and insulin resistance markers (levels of insulin, glucose and HOMA-IR) were measured. From the right ovary sections of 5 to 6 micrometers in diameter were prepared, stained and then studied.Data were analyzed using ANOVA and LSD tests. Results: Hyperglycemia, insulin resistance and reduced body weight of diabetic rats were improved after administration of Abelmoscus esculentus powder in duration of 4 weeks. This effect was relatively similar to metformin. Abelmoscus esculentus powder was caused a significant increase in primordial, primary and graffian follicles (P<0.05) and early corpus luteum. A significant reduction were seen also in the preantral and antral follicle atresia (P<0.05) and old corpus luteum in ovary of diabetic rats. Conclusion: The results of this study showed that Abelmoscus esculentus powder with its antioxidant activity and other properties has a correlation function on hyperglycemia and suitable intermediates for the improvement of ovarian disorders in diabetic rats.
