The radiological study of using fabricated calcium hydroxide from quail eggshell and plasma-rich fibrin for reconstitution of a mandibular bone gap in dogs

Abstract

In this study, Calcium hydroxyl was fabricated from quail egg-shell and autogenous plasma-rich fibrin (PRF) to reconstitute the mandibular gap in dogs. In this study, 27 dogs of both sexes were used, enrolled in three groups, nine of each. A defect as a circular gap experimentally induced on the ventral surface of the lower mandible with a diameter of (14,0.5 mm). Clinical and Radiographical examinations were evaluated at (0,15,30 and 60 days post-surgery), and the XRD (X-Ray Diffractometer), Field Scanning Electron Microscopy (FESEM), and Energy Dispersive X-ray Spectrometer (EDS) analysis were performed. Clinically there was normal mastication and no award complications. Radiographically in 1st group treated with Ca(OH)², the healing near completed, and the opacification of the bone defect in the caudal body of the mandible, with a sclerosed margin representing maturating callus with complete trabecular bridging, whereas in 2nd group at same period representing good maturating callus with complete trabecular bridging, there is disappearance of gap and complete opacification. The XRD scanning of the quail eggshell proved the hexagonal crystalline shape of calcium hydroxide containing essential elements of natural bone calcium, oxygen, and Carbone. At the same time, FESEM demonstrated the characteristic hexagonal shape of the particles, allowing identifying them as calcium hydroxide in Ca(OH)2 group with no porous in PRF. In conclusion, using fabricated calcium hydroxide quail egg shell and prepared autogenous PRF demonstrated an effective bioactive agent with superior biocompatible properties of PRF for reconstitution mandibular defect in dogs; there was increased radiographic density of defective bone.

Keywords

• mandible
• defect
• prf
• quail
• eggshell