Antimicrobial and Cytotoxic Potential of Eucalyptus Essential Oil-Based Nanoemulsions for Mouthwashes Application
Dione Glauco Batista,
William Gustavo Sganzerla,
Lysa Ribeiro da Silva,
Yasmin Gabriele Schmitt Vieira,
Aline R. Almeida,
Diogo Dominguini,
Luciane Ceretta,
Adriana Castro Pinheiro,
Fabiano Cleber Bertoldi,
Daniela Becker,
Dachamir Hotza,
Michael Ramos Nunes,
Cleonice Gonçalves da Rosa,
Anelise Viapiana Masiero
Affiliations
Dione Glauco Batista
Multi-User Laboratory, Graduate Program in Environment and Health, Planalto Catarinense University, Lages 88509-900, SC, Brazil
William Gustavo Sganzerla
School of Food Engineering (FEA), University of Campinas (UNICAMP), Campinas 13083-970, SP, Brazil
Lysa Ribeiro da Silva
Multi-User Laboratory, Graduate Program in Environment and Health, Planalto Catarinense University, Lages 88509-900, SC, Brazil
Yasmin Gabriele Schmitt Vieira
Multi-User Laboratory, Graduate Program in Environment and Health, Planalto Catarinense University, Lages 88509-900, SC, Brazil
Aline R. Almeida
Laboratory of Plasmas, Films, and Surfaces, Santa Catarina State University (UDESC), Joinville 89219-710, SC, Brazil
Diogo Dominguini
Laboratory of Experimental Pathophysiology, Graduate Program in Health Sciences, University of Southern Santa Catarina (UNESC), Criciúma 88806-000, SC, Brazil
Luciane Ceretta
Graduate Program in Collective Health, University of Southern Santa Catarina (UNESC), Criciúma 88806-000, SC, Brazil
Adriana Castro Pinheiro
Center of Chemical, Pharmaceuticals, and Food Sciences, Federal University of Pelotas, Pelotas 96010-610, RS, Brazil
Fabiano Cleber Bertoldi
Agricultural Research and Rural Extension Company of Santa Catarina (EPAGRI), Itajaí 88318-112, SC, Brazil
Daniela Becker
Laboratory of Plasmas, Films, and Surfaces, Santa Catarina State University (UDESC), Joinville 89219-710, SC, Brazil
Dachamir Hotza
Graduate Program in Chemical Engineering (PosENQ), Federal University of Santa Catarina (UFSC), Florianópolis 88040-900, SC, Brazil
Michael Ramos Nunes
Graduate Program in Chemical Engineering (PosENQ), Federal University of Santa Catarina (UFSC), Florianópolis 88040-900, SC, Brazil
Cleonice Gonçalves da Rosa
Multi-User Laboratory, Graduate Program in Environment and Health, Planalto Catarinense University, Lages 88509-900, SC, Brazil
Anelise Viapiana Masiero
Multi-User Laboratory, Graduate Program in Environment and Health, Planalto Catarinense University, Lages 88509-900, SC, Brazil
Objective: An eucalyptus essential oil-based nanoemulsion was produced and evaluated for its antimicrobial properties against Streptococcus mutans and its cytotoxicity in the surface mucous cells of rabbits. Methods: The essential oil-based nanoemulsion was synthesized with two species of eucalyptus—Eucalyptus citriodora and Eucalyptus globulus—followed by physicochemical characterization and the determination of antimicrobial activity and cell viability. Subsequently, the mouthwash formulations (fluoride and fluoride-free) were functionalized with the nanoemulsion, and their in vitro antimicrobial actions were evaluated against S. mutans. Results: The nanoemulsion presented an average particle size of around 100 nm, a polydispersity index close to 0.3, a zeta potential between −19 and −30 mV, a pH close to 7, a spherical shape, and a cell viability above 50%. The antimicrobial activity analysis showed that the nanoemulsion was effective in the control of S. mutans. The mouthwashes functionalized with the nanoemulsion also presented bacteriostatic and bactericidal properties. Conclusions: The bio-based material produced with eucalyptus essential oil presented adequate physicochemical characteristics, with the potential to be used as an innovative material in preventive dentistry, contributing to the maintenance of oral and systemic health.
