In this study, the vaccination coverage, serological sampling and infection rate of sheep and goats were evaluated as predictors for the modeling of human brucellosis in Greece. The human brucellosis disease frequency per local regional unit (RU) varied significantly (RR90) among consecutive years. The notification rate was higher (p p = 0.03) and higher vaccination coverage of the animals (estimate: −0.349 (−0.72; −0.07), p p = 0.001), limiting the reliable estimation of the infection rate in livestock and the design of an integrated One Health model for human disease.