Generation of Mt3 Homozygote murine ES cell lines via CRISPR/Cas9 technology

Huanxin Zhou; Hongyan Sun; Zhijiang Rong; Wei Cui

Stem Cell Research (Apr 2022)

Generation of Mt3 Homozygote murine ES cell lines via CRISPR/Cas9 technology

Huanxin Zhou,
Hongyan Sun,
Zhijiang Rong,
Wei Cui

Affiliations

Huanxin Zhou: College of Chemical and Biological Engineering, Taiyuan University of Science and Technology, No.66 Waliu Road, Taiyuan 030024, PR China; Corresponding author at: College of Chemical and Biological Engineering, Taiyuan University of Science and Technology, No.66 Waliu Road, Taiyuan 030024, PR China.
Hongyan Sun: College of Chemical and Biological Engineering, Taiyuan University of Science and Technology, No.66 Waliu Road, Taiyuan 030024, PR China
Zhijiang Rong: College of Chemical and Biological Engineering, Taiyuan University of Science and Technology, No.66 Waliu Road, Taiyuan 030024, PR China
Wei Cui: Shanxi Academy of Environmental Sciences, 11 Xinghua Street, Taiyuan 030000, PR China

Journal volume & issue: Vol. 60
p. 102714

Abstract

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Metallothioneins belong to a superfamily of intracellular metal-binding proteins, have bearing on almost all biochemical processes,include free radical scavenging, apoptosis, regulation of intracellular redox balance, and anti-inflammatory processes. By using a CRISPR/Cas 9 system, we generated Mt3-/- mES line. This cell line has contributed to further investigation of the functions of Mtt3 during early development, as well as a cell model for screening for metal-related detoxificationan and antioxidant response phenotype during stem cell differentiation.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

About the journal