Biofilm-forming bacteria and their antibiotic resistance in treated water supplies in Ilorin Metropolis, Nigeria

Abstract

Several water-related disease outbreaks have been attributed to deficiencies in water distribution systems (WDS). This study determined the relationship between biofilm-producing bacteria and their antibiotic resistance in WDS in Ilorin Metropolis, Nigeria through the assessment of the quality of water, the virulence factors possessed and susceptibility to some antibiotics. The physicochemical and bacteriological quality of the water samples were analyzed using standard methods. A total of 60 samples were collected from seven different locations within Ilorin metropolis. The pH of the water samples ranged from 6.58 – 7.22, turbidity 0.14 – 2.07 NTU and free residual chlorine ranged from 0.00 – 1.14 mg/L. A total of eleven bacteria isolates were identified by biochemical and molecular methods. They included genera of Pseudomonas, Escherichia, Staphylococcus, Klebsiella, Citrobacter, Shigella, Proteus, Yersinia and Serratia. The total bacterial counts were 1.10 – 7.20 × 102 cfu/mL. The isolates consisted of 72.7% biofilm producers using the tube method and 63.6% biofilm producers using the congo red method. Both biofilm and non-biofilm isolates displayed complete resistance to ceftriazone, amoxicillin, tetracycline, and cotrimoxazole. Biofilm producers showed stronger relationship with antibiotic resistance (p < 0.0001) than non-biofilms, thus suggesting the use of bacterial biofilm counts as indicators of water quality.

Keywords

• biofilm, potable water, virulence, antibiotic resistance, water distribution systems