Bisphosphonate-adsorbed ceramic nanoparticles increase bone formation in an injectable carrier for bone tissue engineering

Tegan L Cheng; Ciara M Murphy; Roya Ravarian; Fariba Dehghani; David G Little; Aaron Schindeler

doi:10.1177/2041731415609448

Journal of Tissue Engineering (Oct 2015)

Bisphosphonate-adsorbed ceramic nanoparticles increase bone formation in an injectable carrier for bone tissue engineering

Tegan L Cheng,
Ciara M Murphy,
Roya Ravarian,
Fariba Dehghani,
David G Little,
Aaron Schindeler

Affiliations

Tegan L Cheng: Discipline of Paediatrics and Child Health, Sydney Medical School, University of Sydney, Sydney, NSW, Australia
Ciara M Murphy: Discipline of Paediatrics and Child Health, Sydney Medical School, University of Sydney, Sydney, NSW, Australia
Roya Ravarian: School of Chemical and Biomolecular Engineering, University of Sydney, Sydney, NSW, Australia
Fariba Dehghani: School of Chemical and Biomolecular Engineering, University of Sydney, Sydney, NSW, Australia
David G Little: Discipline of Paediatrics and Child Health, Sydney Medical School, University of Sydney, Sydney, NSW, Australia
Aaron Schindeler: Discipline of Paediatrics and Child Health, Sydney Medical School, University of Sydney, Sydney, NSW, Australia

DOI: https://doi.org/10.1177/2041731415609448
Journal volume & issue: Vol. 6

Abstract

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Sucrose acetate isobutyrate (SAIB) is a sugar-based carrier. We have previously applied SAIB as a minimally invasive system for the co-delivery of recombinant human bone morphogenetic protein-2 (rhBMP-2) and found synergy when co-delivering zoledronic acid (ZA) and hydroxyapatite (HA) nanoparticles. Alternative bioceramics were investigated in a murine SAIB/rhBMP-2 injection model. Neither beta-tricalcium phosphate (TCP) nor Bioglass (BG) 45S5 had a significant effect on bone volume (BV) alone or in combination with the ZA. 14 C-labelled ZA binding assays showed particle size and ceramic composition affected binding with nano-HA > micro-HA > TCP > BG. Micro-HA and nano-HA increased BV in a rat model of rhBMP-2/SAIB injection (+278% and +337%), and BV was further increased with ZA–adsorbed micro-HA and nano-HA (+530% and +889%). These data support the use of ZA–adsorbed nanoparticle-sized HA as an optimal additive for the SAIB/rhBMP-2 injectable system for bone tissue engineering.

Published in Journal of Tissue Engineering

ISSN: 2041-7314 (Online)
Publisher: SAGE Publishing
Country of publisher: United Kingdom
LCC subjects: Science: Chemistry: Organic chemistry: Biochemistry
Website: https://journals.sagepub.com/home/tej

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