Critical Defect Healing Assessment in Rat Calvaria Filled with Injectable Calcium Phosphate Cement

Luis  Eduardo Schmidt; Henrique Hadad; Igor  Rodrigues de Vasconcelos; Luara  Teixeira Colombo; Rodrigo  Capalbo da Silva; Ana  Flavia Piquera Santos; Lara  Cristina Cunha Cervantes; Pier  Paolo Poli; Fabrizio Signorino; Carlo Maiorana; Paulo  Sérgio Perri de Carvalho; Francisley  Ávila Souza

doi:10.3390/jfb10020021

Journal of Functional Biomaterials (May 2019)

Critical Defect Healing Assessment in Rat Calvaria Filled with Injectable Calcium Phosphate Cement

Luis Eduardo Schmidt,
Henrique Hadad,
Igor Rodrigues de Vasconcelos,
Luara Teixeira Colombo,
Rodrigo Capalbo da Silva,
Ana Flavia Piquera Santos,
Lara Cristina Cunha Cervantes,
Pier Paolo Poli,
Fabrizio Signorino,
Carlo Maiorana,
Paulo Sérgio Perri de Carvalho,
Francisley Ávila Souza

Affiliations

Luis Eduardo Schmidt: Implant Dentistry Post-Graduation Program, São Leopoldo Mandic School of Dentistry and Research Center, Campinas 13.045-755, Brazil
Henrique Hadad: Department of Surgery and Integrated Clinic, Araçatuba Dental of School, São Paulo State University Júlio de Mesquita Filho—UNESP, Araçatuba, São Paulo 16.015.050, Brazil
Igor Rodrigues de Vasconcelos: Implant Dentistry Post-Graduation Program, São Leopoldo Mandic School of Dentistry and Research Center, Campinas 13.045-755, Brazil
Luara Teixeira Colombo: Department of Surgery and Integrated Clinic, Araçatuba Dental of School, São Paulo State University Júlio de Mesquita Filho—UNESP, Araçatuba, São Paulo 16.015.050, Brazil
Rodrigo Capalbo da Silva: Department of Surgery and Integrated Clinic, Araçatuba Dental of School, São Paulo State University Júlio de Mesquita Filho—UNESP, Araçatuba, São Paulo 16.015.050, Brazil
Ana Flavia Piquera Santos: Department of Surgery and Integrated Clinic, Araçatuba Dental of School, São Paulo State University Júlio de Mesquita Filho—UNESP, Araçatuba, São Paulo 16.015.050, Brazil
Lara Cristina Cunha Cervantes: Department of Surgery and Integrated Clinic, Araçatuba Dental of School, São Paulo State University Júlio de Mesquita Filho—UNESP, Araçatuba, São Paulo 16.015.050, Brazil
Pier Paolo Poli: Implant Center for Edentulism and Jawbone Atrophies, Maxillofacial Surgery and Odontostomatology Unit, Fondazione IRCSS Cà Granda Maggiore Policlinico Hospital, University of Milan, 47.031 Milan, Italy
Fabrizio Signorino: Implant Center for Edentulism and Jawbone Atrophies, Maxillofacial Surgery and Odontostomatology Unit, Fondazione IRCSS Cà Granda Maggiore Policlinico Hospital, University of Milan, 47.031 Milan, Italy
Carlo Maiorana: Implant Center for Edentulism and Jawbone Atrophies, Maxillofacial Surgery and Odontostomatology Unit, Fondazione IRCSS Cà Granda Maggiore Policlinico Hospital, University of Milan, 47.031 Milan, Italy
Paulo Sérgio Perri de Carvalho: Implant Dentistry Post-Graduation Program, São Leopoldo Mandic School of Dentistry and Research Center, Campinas 13.045-755, Brazil
Francisley Ávila Souza: Department of Surgery and Integrated Clinic, Araçatuba Dental of School, São Paulo State University Júlio de Mesquita Filho—UNESP, Araçatuba, São Paulo 16.015.050, Brazil

DOI: https://doi.org/10.3390/jfb10020021
Journal volume & issue: Vol. 10, no. 2
p. 21

Abstract

Read online

(1) Background: The tissue engineering field has been working to find biomaterials that mimic the biological properties of autogenous bone grafts. (2) Aim: To evaluate the osteoconduction potential of injectable calcium phosphate cement implanted in critical defects in rat calvaria. (3) Methods: In the calvarial bone of 36 rats, 7-mm diameter critical size defects were performed. Afterwards, the animals were randomly divided into three groups according to filler material: a blood clot group (BC), blood clot membrane group (BCM), and an injectable β-tricalcium phosphate group (HBS) cement group. After periods of 30 and 60 days, the animals were euthanized, the calvaria was isolated, and submitted to a decalcification process for later blades confection. Qualitative and quantitative analysis of the neoformed bone tissue were conducted, and histometric data were statistically analyzed. (4) Results: Sixty days post-surgery, the percentages of neoformed bone were 10.67 ± 5.57 in group BC, 16.71 ± 5.0 in group BCM, and 55.11 ± 13.20 in group HBS. The bone formation values in group HBS were significantly higher (p < 0.05) than in groups BC and BCM. (5) Conclusions: Based on these results, it can be concluded that injectable calcium phosphate cement is an osteoconductive material that can be used to fill bone cavities.

Published in Journal of Functional Biomaterials

ISSN: 2079-4983 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Technology: Chemical technology: Biotechnology; Medicine: Medicine (General)
Website: http://www.mdpi.com/journal/jfb/

About the journal

Abstract

Keywords