In vitro analysis of the phytotoxic and genotoxic potential of Aligarh wastewater and Mathura refinery wastewater

Abstract

Present report deals with the phytotoxicity and genotoxicity of Mathura refinery wastewater and Aligarh wastewater of Northern India. The IC50 value in Allium cepa root growth inhibition test was recorded to be 0.14X and 0.10X for Mathura refinery and Aligarh industrial wastewaters, respectively. Significant decline in the survival of various Escherichia coli K12 DNA repair defective mutants was observed when the tester strains were exposed to the aforementioned samples. The order of sensitivity was invariably as: AB1157 (wild type) < AB2494 (lexA mutant) < AB2463 (recA mutant) < AB2480 (uvrA recA double mutant). These results suggested a significant amount of DNA damage within the bacterial cells exposed to test wastewaters. A. cepa genotoxicity test also demonstrated a considerable amount of chromosomal damage of A. cepa brought about by the test samples. The aberration index (A.I.) for Aligarh wastewater and refinery wastewater was recorded to be 11.2% and 14.7%, respectively, whereas the aquaguard mineral water serving as negative control displayed the A.I. value to be 2.6%. Interestingly, genotoxicity of both industrial wastewaters was reduced to a remarkable extent in presence of mannitol, the hydroxyl radical scavenger. Present study clearly indicated a distinct pattern of the chromosomal aberrations showing predominantly stickiness and stray chromosomes in case of AWW while clumping and stickiness in case of RWW, thereby affirming the genotoxicity of both test waters.

Keywords

• Allium cepa test
• Chromosomal aberration
• Escherichia coli survival
• Genotoxicity testing
• Hydroxyl radical scavenger
• IC50 value