Relationship between puerarin and urothelial carcinoembryonic antigen 1 expression in apoptosis and autophagy of esophageal cancer cells

Abstract

"Objective To explore the molecular mechanism of puerarin on apoptosis and autophagy in esophageal cancer cells. Methods Human esophageal cancer EC109 cells were treated with puerarin at 0, 200, 500, 800 μmol/L. The cell proliferation,migration and invasion ability were measured by CCK-8 method, the scratch assay and transwell test, respectively. The expression levels of apoptosis-related proteins (pro-apoptotic proteins Bax and Bad, anti-apoptotic protein Bcl-2) and autophagy-related proteins p62, Beclin-1 and ATG5 were detected by Western blot. The expression level of urothelial carcinoembryonic antigen 1 (UCA1) was detected by real-time fluorescence quantitative polymerase chain reaction. EC109 cell lines stably overexpressing UCA1 were constructed by lentiviral transfection and divided into stable overexpressing UCA1 cells (UCA1-OE group) and negative control cells(UCA1-OENC group). The two groups of cells were treated with the same 500 μmol/L puerarin, and the expression levels of cells apoptosis and autophagy protein were detected using cellular immunofluorescence assay. Results Puerarin treatment with 200, 500 and 800 μmol/L significantly inhibited the proliferation, migration and invasion activities of EC109 cells ( P Conclusion Puerarin induces apoptosis and autophagy in esophageal cancer cells by inhibiting the expression of UCA1."

Keywords

• puerarin
• urothelial carcinoembryonic antigen 1
• esophageal cancer
• apoptosis
• autophagy