Extracellular vesicles from adipose stem cells ameliorate allergic rhinitis in mice by immunomodulatory

Wenhan Yang; Wenhan Yang; Zhiyu Pan; Jiacheng Zhang; Lian Wang; Ju lai; Ju lai; Shican Zhou; Shican Zhou; Zhili Zhang; Kai Fan; Dan Deng; Dan Deng; Zhengliang Gao; Zhengliang Gao; Zhengliang Gao; Shaoqing Yu; Shaoqing Yu

doi:10.3389/fimmu.2023.1302336

Frontiers in Immunology (Dec 2023)

Extracellular vesicles from adipose stem cells ameliorate allergic rhinitis in mice by immunomodulatory

Wenhan Yang,
Wenhan Yang,
Zhiyu Pan,
Jiacheng Zhang,
Lian Wang,
Ju lai,
Ju lai,
Shican Zhou,
Shican Zhou,
Zhili Zhang,
Kai Fan,
Dan Deng,
Dan Deng,
Zhengliang Gao,
Zhengliang Gao,
Zhengliang Gao,
Shaoqing Yu,
Shaoqing Yu

Affiliations

Wenhan Yang: Department of Otorhinolaryngology Head and Neck Surgery, Tongji Hospital, School of Medicine, Tongji University, Shanghai, China
Wenhan Yang: School of Medicine, Tongji University, Shanghai, China
Zhiyu Pan: Department of Otorhinolaryngology Head and Neck Surgery, Tongji Hospital, School of Medicine, Tongji University, Shanghai, China
Jiacheng Zhang: Department of Otorhinolaryngology Head and Neck Surgery, Tongji Hospital, School of Medicine, Tongji University, Shanghai, China
Lian Wang: School of Medicine, Tongji University, Shanghai, China
Ju lai: Department of Otorhinolaryngology Head and Neck Surgery, Tongji Hospital, School of Medicine, Tongji University, Shanghai, China
Ju lai: School of Medicine, Tongji University, Shanghai, China
Shican Zhou: Department of Otorhinolaryngology Head and Neck Surgery, Tongji Hospital, School of Medicine, Tongji University, Shanghai, China
Shican Zhou: School of Medicine, Tongji University, Shanghai, China
Zhili Zhang: Department of Otorhinolaryngology Head and Neck Surgery, Tongji Hospital, School of Medicine, Tongji University, Shanghai, China
Kai Fan: Department of Otorhinolaryngology Head and Neck Surgery, Tongji Hospital, School of Medicine, Tongji University, Shanghai, China
Dan Deng: Department of Dermatology, Shanghai Children’s Medical Center, School of Medicine, Shanghai Jiao Tong University, Shanghai, China
Dan Deng: Department of Dermatology, Xinhua Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China
Zhengliang Gao: Fundamental Research Center, Shanghai YangZhi Rehabilitation Hospital (Shanghai Sunshine Rehabilitation Center), School of Medicine, Tongji University, Shanghai, China
Zhengliang Gao: Shanghai Engineering Research Center of Organ Repair, School of Medicine, Shanghai University, Shanghai, China
Zhengliang Gao: Institute of Geriatrics (Shanghai University), Affiliated Nantong Hospital of Shanghai University (The Sixth People’s Hospital of Nantong), School of Medicine, Shanghai University, Nantong, China
Shaoqing Yu: Department of Otorhinolaryngology Head and Neck Surgery, Tongji Hospital, School of Medicine, Tongji University, Shanghai, China
Shaoqing Yu: Department of Allergy, Tongji Hospital, School of Medicine, Tongji University, Shanghai, China

DOI: https://doi.org/10.3389/fimmu.2023.1302336
Journal volume & issue: Vol. 14

Abstract

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BackgroundHuman adipose tissue-derived stem cells (hADSCs) exert potent immunosuppressive effects in the allogeneic transplantation treatment. In mouse model of allergic rhinitis (AR), ADSCs partially ameliorated AR. However, no study has evaluated the potential therapeutic effects of hADSC-derived extracellular vesicles (hADSC-EVs) on AR.MethodsFemale BALB/c mice were sensitized and challenged with ovalbumin (OVA) to induce AR. One day after the last nasal drop, each group received phosphate buffered saline (PBS) or hADSC-EVs treatment. Associated symptoms and biological changes were then assessed.ResultshADSC-EV treatment significantly alleviated nasal symptoms, and reduced inflammatory infiltration. Serum levels of OVA-specific IgE, interleukin (IL)-4 and interferon (IFN)-γ were all significantly reduced. The mRNA levels of IL-4 and IFN-γ in the spleen also changed accordingly. The T helper (Th)1/Th2 cell ratio increased. The treatment efficacy index of hADSC-EV was higher than that of all human-derived MSCs in published reports on MSC treatment of AR. ADSC-EVs exhibited a greater therapeutic index in most measures when compared to our previous treatment involving ADSCs.ConclusionThese results demonstrated that hADSC-EVs could ameliorate the symptoms of AR by modulating cytokine secretion and Th1/Th2 cell balance. hADSC-EVs could potentially be a viable therapeutic strategy for AR. Further animal studies are needed to elucidate the underlying mechanisms and to optimize potential clinical protocols.

Published in Frontiers in Immunology

ISSN: 1664-3224 (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Medicine: Internal medicine: Specialties of internal medicine: Immunologic diseases. Allergy
Website: http://journal.frontiersin.org/journal/immunology

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