Diversity of Mitochondrial DNA Haplogroups and Their Association with Bovine Antral Follicle Count
Hongfei Liu,
Junjun Zhai,
Hui Wu,
Jingyi Wang,
Shaowei Zhang,
Jie Li,
Zhihan Niu,
Chenglong Shen,
Kaijuan Zhang,
Zhengqing Liu,
Fugui Jiang,
Enliang Song,
Xiuzhu Sun,
Yongsheng Wang,
Xianyong Lan
Affiliations
Hongfei Liu
College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China
Junjun Zhai
College of Life Science, Yulin University, Yulin 719000, China
Hui Wu
College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China
Jingyi Wang
College of Veterinary Medicine, Northwest A&F University, Yangling 712100, China
Shaowei Zhang
College of Veterinary Medicine, Northwest A&F University, Yangling 712100, China
Jie Li
College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China
Zhihan Niu
College of Veterinary Medicine, Northwest A&F University, Yangling 712100, China
Chenglong Shen
College of Veterinary Medicine, Northwest A&F University, Yangling 712100, China
Kaijuan Zhang
College of Veterinary Medicine, Northwest A&F University, Yangling 712100, China
Zhengqing Liu
College of Veterinary Medicine, Northwest A&F University, Yangling 712100, China
Fugui Jiang
Shandong Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan 250000, China
Enliang Song
Shandong Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan 250000, China
Xiuzhu Sun
College of Grassland Agriculture, Northwest A&F University, Yangling 712100, China
Yongsheng Wang
College of Veterinary Medicine, Northwest A&F University, Yangling 712100, China
Xianyong Lan
College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China
Maternal origins based on the bovine mitochondrial D-loop region are proven to have two main origins: Bos taurus and Bos indicus. To examine the association between the maternal origins of bovine and reproductive traits, the complete mitochondrial D-loop region sequences from 501 Chinese Holstein cows and 94 individuals of other breeds were analyzed. Based on the results obtained from the haplotype analysis, 260 SNPs (single nucleotide polymorphism), 32 indels (insertion/deletion), and 219 haplotypes were identified. Moreover, the nucleotide diversity (π) and haplotype diversity (Hd) were 0.024 ± 0.001 and 0.9794 ± 0.003, respectively, indicating the abundance of genetic resources in Chinese Holstein cows. The results of the median-joining network analysis showed two haplogroups (HG, including HG1 and HG2) that diverged in genetic distance. Furthermore, the two haplogroups were significantly (p Bos indicus and Bos taurus, respectively. Therefore, HG1 originating from the maternal ancestors of Bos indicus may have a greater reproductive performance, and potential genetic variants discovered may promote the breeding process in the cattle industry.
