Nature Communications (Apr 2017)
Direct comparison of distinct naive pluripotent states in human embryonic stem cells
- S. Warrier,
- M. Van der Jeught,
- G. Duggal,
- L. Tilleman,
- E. Sutherland,
- J. Taelman,
- M. Popovic,
- S. Lierman,
- S. Chuva De Sousa Lopes,
- A. Van Soom,
- L. Peelman,
- F. Van Nieuwerburgh,
- D. I. M. De Coninck,
- B. Menten,
- P. Mestdagh,
- J. Van de Sompele,
- D. Deforce,
- P. De Sutter,
- B. Heindryckx
Affiliations
- S. Warrier
- Department for Reproductive Medicine, Ghent-Fertility and Stem cell Team (G-FaST), Ghent University Hospital
- M. Van der Jeught
- Department for Reproductive Medicine, Ghent-Fertility and Stem cell Team (G-FaST), Ghent University Hospital
- G. Duggal
- Department for Reproductive Medicine, Ghent-Fertility and Stem cell Team (G-FaST), Ghent University Hospital
- L. Tilleman
- Department of Pharmaceutics, Laboratory for Pharmaceutical Biotechnology, Faculty of Pharmaceutical Sciences, Ghent University
- E. Sutherland
- Department for Reproductive Medicine, Ghent-Fertility and Stem cell Team (G-FaST), Ghent University Hospital
- J. Taelman
- Department for Reproductive Medicine, Ghent-Fertility and Stem cell Team (G-FaST), Ghent University Hospital
- M. Popovic
- Department for Reproductive Medicine, Ghent-Fertility and Stem cell Team (G-FaST), Ghent University Hospital
- S. Lierman
- Department for Reproductive Medicine, Ghent-Fertility and Stem cell Team (G-FaST), Ghent University Hospital
- S. Chuva De Sousa Lopes
- Department for Reproductive Medicine, Ghent-Fertility and Stem cell Team (G-FaST), Ghent University Hospital
- A. Van Soom
- Department of Reproduction, Obstetrics and Herd Health, Faculty of Veterinary Medicine, Ghent University
- L. Peelman
- Department of Nutrition, Genetics and Ethology, Faculty of Veterinary Medicine, Ghent University
- F. Van Nieuwerburgh
- Department of Pharmaceutics, Laboratory for Pharmaceutical Biotechnology, Faculty of Pharmaceutical Sciences, Ghent University
- D. I. M. De Coninck
- Department of Pharmaceutics, Laboratory for Pharmaceutical Biotechnology, Faculty of Pharmaceutical Sciences, Ghent University
- B. Menten
- Department of Pediatrics and Medical Genetics, Center for Medical Genetics, Ghent University Hospital
- P. Mestdagh
- Department of Pediatrics and Medical Genetics, Center for Medical Genetics, Ghent University Hospital
- J. Van de Sompele
- Department of Pediatrics and Medical Genetics, Center for Medical Genetics, Ghent University Hospital
- D. Deforce
- Department of Pharmaceutics, Laboratory for Pharmaceutical Biotechnology, Faculty of Pharmaceutical Sciences, Ghent University
- P. De Sutter
- Department for Reproductive Medicine, Ghent-Fertility and Stem cell Team (G-FaST), Ghent University Hospital
- B. Heindryckx
- Department for Reproductive Medicine, Ghent-Fertility and Stem cell Team (G-FaST), Ghent University Hospital
- DOI
- https://doi.org/10.1038/ncomms15055
- Journal volume & issue
-
Vol. 8,
no. 1
pp. 1 – 10
Abstract
Human embryonic stem cells (hESCs) in culture display a state of primed pluripotency, but recent protocols have been developed that enable hESCs to adopt a naive-like pluripotent state. Here the authors perform a side-by-side comparison of methods used to culture naive hESCs and confirm the role of PI3K/AKT/mTORC signalling in facilitating the induction of naive pluripotency.
