Protective Immunity Elicited by VP1 Chimeric Antigens of Bacterial Ghosts against Hand-Foot-and-Mouth Disease Virus
Saisai Gong,
Nan Nan,
Yakun Sun,
Zhili He,
Jiajia Li,
Fanghong Chen,
Tao Li,
Nianzhi Ning,
Jianxin Wang,
Zhan Li,
Deyan Luo,
Hui Wang
Affiliations
Saisai Gong
State Key Laboratory of Pathogen and Biosecurity,Beijing Institute of Microbiology and Epidemiology, 20 Dongda Street, Fengtai District, Beijing 100071, China
Nan Nan
State Key Laboratory of Pathogen and Biosecurity,Beijing Institute of Microbiology and Epidemiology, 20 Dongda Street, Fengtai District, Beijing 100071, China
Yakun Sun
State Key Laboratory of Pathogen and Biosecurity,Beijing Institute of Microbiology and Epidemiology, 20 Dongda Street, Fengtai District, Beijing 100071, China
Zhili He
State Key Laboratory of Pathogen and Biosecurity,Beijing Institute of Microbiology and Epidemiology, 20 Dongda Street, Fengtai District, Beijing 100071, China
Jiajia Li
State Key Laboratory of Pathogen and Biosecurity,Beijing Institute of Microbiology and Epidemiology, 20 Dongda Street, Fengtai District, Beijing 100071, China
Fanghong Chen
State Key Laboratory of Pathogen and Biosecurity,Beijing Institute of Microbiology and Epidemiology, 20 Dongda Street, Fengtai District, Beijing 100071, China
Tao Li
State Key Laboratory of Pathogen and Biosecurity,Beijing Institute of Microbiology and Epidemiology, 20 Dongda Street, Fengtai District, Beijing 100071, China
Nianzhi Ning
State Key Laboratory of Pathogen and Biosecurity,Beijing Institute of Microbiology and Epidemiology, 20 Dongda Street, Fengtai District, Beijing 100071, China
Jianxin Wang
State Key Laboratory of Pathogen and Biosecurity,Beijing Institute of Microbiology and Epidemiology, 20 Dongda Street, Fengtai District, Beijing 100071, China
Zhan Li
State Key Laboratory of Pathogen and Biosecurity,Beijing Institute of Microbiology and Epidemiology, 20 Dongda Street, Fengtai District, Beijing 100071, China
Deyan Luo
State Key Laboratory of Pathogen and Biosecurity,Beijing Institute of Microbiology and Epidemiology, 20 Dongda Street, Fengtai District, Beijing 100071, China
Hui Wang
State Key Laboratory of Pathogen and Biosecurity,Beijing Institute of Microbiology and Epidemiology, 20 Dongda Street, Fengtai District, Beijing 100071, China
This study was designed to evaluate the immunogenicity and protective efficacy of two VP1 chimeric antigens of bacterial ghosts. Inoculation of the two VP1 chimeric antigens of bacterial ghosts into BALB/c mice markedly elicited humoral and mucosal immune responses. The specific antibodies induced by the chimeric ghosts protected mice not only against the virus that causes hand-foot-and-mouth disease but also against E. coli O157:H7 bacterial infection. In comparison with the negative control, immunization with the chimeric ghosts protected mice against two LD50 hand-foot-and-mouth disease viral infection. In addition, this specific immunity also protected the pups of pregnant mice immunized with the VP1 chimeric antigens of bacterial ghosts against 20 MLD E. coli O157:H7 infection. Taken together, the results of this study verify for the first time that the VP1 chimeric antigens of bacterial ghosts are target candidates for a new type of vaccine against hand-foot-and-mouth disease. Additionally, this vaccine strategy also elicited a stronger immune response against E. coli O157:H7.
