Molecules (Jun 2024)

Chemical Synthesis and Insecticidal Activity Research Based on α-Conotoxins

  • Chengzhang Lin,
  • Hailong Qin,
  • Yanling Liao,
  • Jiao Chen,
  • Bingmiao Gao

DOI
https://doi.org/10.3390/molecules29122846
Journal volume & issue
Vol. 29, no. 12
p. 2846

Abstract

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The escalating resistance of agricultural pests to chemical insecticides necessitates the development of novel, efficient, and safe biological insecticides. Conus quercinus, a vermivorous cone snail, yields a crude venom rich in peptides for marine worm predation. This study screened six α-conotoxins with insecticidal potential from a previously constructed transcriptome database of C. quercinus, characterized by two disulfide bonds. These conotoxins were derived via solid-phase peptide synthesis (SPPS) and folded using two-step iodine oxidation for further insecticidal activity validation, such as CCK-8 assay and insect bioassay. The final results confirmed the insecticidal activities of the six α-conotoxins, with Qc1.15 and Qc1.18 exhibiting high insecticidal activity. In addition, structural analysis via homology modeling and functional insights from molecular docking offer a preliminary look into their potential insecticidal mechanisms. In summary, this study provides essential references and foundations for developing novel insecticides.

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