First report on the association of a begomovirus with chrysanthemum indicum exhibiting yellowing of leaf vein disease characterized by molecular studies

Abstract

Infected leaf samples of an ornamental plant Chrysanthemum indicum showing yellowing of leaf veins were collected from gardens of New Delhi (India). An expected PCR product of size ~500 bp was amplified from total DNA extracts of symptomatic leaf samples with universal primers on the gene of coat protein region of begomovirus DNA-A component. The presence of begomoviruses was also confirmed by Southern blot analysis using control cloned DNA-A probe of Cotton leaf curl virus. Sequence analysis of the virus infecting Chrysanthemum indicum showed 99% nucleotide sequence identity with Clerodendron yellow mosaic virus (EF408037).

Keywords

• chrysanthemum indicum plants
• begomovirus
• pcr
• sequencing
• southern blot