Current Issues in Molecular Biology (Jun 2023)

Biochemical and Computational Assessment of Acute Phase Proteins in Dairy Cows Affected with Subclinical Mastitis

  • Aarif Ali,
  • Muneeb U. Rehman,
  • Saima Mushtaq,
  • Sheikh Bilal Ahmad,
  • Altaf Khan,
  • Anik Karan,
  • Amir Bashir Wani,
  • Showkat Ahmad Ganie,
  • Manzoor Ur Rahman Mir

DOI
https://doi.org/10.3390/cimb45070338
Journal volume & issue
Vol. 45, no. 7
pp. 5317 – 5346

Abstract

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Subclinical mastitis (SCM) is a predominant form of mastitis wherein major visible signs of disease are absent. The present study aimed to determine acute phase proteins (APPs) like ferritin, C-reactive protein (CRP), and microalbumin (Malb) in 135 composite milk and serum samples of healthy (n = 25) and SCM (n = 110) cows. As bovine mastitis is an inflammatory disease, the present study also aimed at finding novel anti-inflammatory compounds from natural sources by repurposing approach using computational studies. The findings of the present study revealed substantial elevation (p p p p p p p p > 0.05), and Malb (r = 0.16, p > 0.05). The findings of molecular docking revealed that Chaetoglobosin U was the most effective molecule that showed the highest binding affinity (kcal/mol) of −10.1 and −8.5 against ferritin and albumin. The present study concluded that the estimation of cow-side tests, SCC, and APPs in milk/serum is suitable to detect SCM and screening herd community. Furthermore, Chaetoglobosin U could be developed as a promising anti-inflammatory inhibitor; however, further studies are required to validate these findings.

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