Detailed data from experimentally-induced mastitis in ewes, with the aim to evaluate cathelicidin-1 in milk
Angeliki I. Katsafadou,
George Th. Tsangaris,
Natalia G.C. Vasileiou,
Katerina S. Ioannidi,
Athanasios K. Anagnostopoulos,
Charalambos Billinis,
Ilektra A. Fragkou,
Elias Papadopoulos,
Vasia S. Mavrogianni,
Charalambia K. Michael,
M. Filippa Addis,
George C. Fthenakis
Affiliations
Angeliki I. Katsafadou
Veterinary Faculty, University of Thessaly, 43100 Karditsa, Greece; Proteomics Research Unit, Biomedical Research Foundation of Academy of Athens, 11527 Athens, Greece
George Th. Tsangaris
Proteomics Research Unit, Biomedical Research Foundation of Academy of Athens, 11527 Athens, Greece
Natalia G.C. Vasileiou
Veterinary Faculty, University of Thessaly, 43100 Karditsa, Greece
Katerina S. Ioannidi
Veterinary Faculty, University of Thessaly, 43100 Karditsa, Greece
Athanasios K. Anagnostopoulos
Proteomics Research Unit, Biomedical Research Foundation of Academy of Athens, 11527 Athens, Greece
Charalambos Billinis
Veterinary Faculty, University of Thessaly, 43100 Karditsa, Greece
Ilektra A. Fragkou
Veterinary Faculty, University of Thessaly, 43100 Karditsa, Greece
Elias Papadopoulos
Laboratory of Parasitology and Parasitic Diseases, Faculty of Veterinary Medicine, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece
Vasia S. Mavrogianni
Veterinary Faculty, University of Thessaly, 43100 Karditsa, Greece
Charalambia K. Michael
Veterinary Faculty, University of Thessaly, 43100 Karditsa, Greece
M. Filippa Addis
Dipartimento di Medicina Veterinaria, Università degli Studi di Milano, Via G. Celoria 10, 20133 Milan, Italy
George C. Fthenakis
Veterinary Faculty, University of Thessaly, 43100 Karditsa, Greece; Corresponding author.
Bacteriological, cytological and proteomics data have been obtained from ewes in two experiments, after intramammary challenge with Mannheimia haemolytica or Staphylococcus chromogenes. Animals were sampled before and sequentially after challenge. Conventional techniques were employed for bacterial isolation and somatic cell counting in milk samples; milk whey samples were subjected to proteomics evaluation by using two-dimensional gel electrophoresis and MALDI-TOF mass spectrometry. There was a correlation between leucocyte content and cathelicidin-1 spot densities in milk samples, although the protein was detected in milk earlier than the increase in leucocyte content. There was also a significant association between presence of mastitis in a mammary gland and detection of cathelicidin-1 in the respective milk sample; the degree of association was greater during the first 24 h post-inoculation. The data are further discussed in the research article “Detection of cathelicidin-1 in the milk as an early indicator of mastitis in ewes” [1]. Keywords: Biomarker, Diagnosis, Mastitis, Sheep, Somatic cell counts
