Development of a porcine reproductive and respiratory syndrome virus colloidal gold detection strip

Abstract

Porcine reproductive and respiratory syndrome virus (PRRSV) is one of the most important causative agents to the worldwide swine industry, caused significant economic losses annually. Here, we aims to prepare a colloidal gold cards for PRRSV early screening. A double-antibody sandwich colloidal gold cards were optimized and prepared using recombinant PRRSV antigen and its paired monoclonal antibody. The test performance of the colloidal gold cards were evaluated using positive and negative samples. We successfully prepared the colloidal gold cards for fast screening of PRRSV. The colloidal gold cards has no cross reaction with various serum samples, showed high specificity. The detection sensitivity reached 1:800. The colloidal gold cards can be stably stored at 37°C for 7 days without any decrease in sensitivity. At 1:800 dilution, the repeatability was still good. The prepared colloidal gold cards has high specificity, sensitivity, stability and repeatability, it provided an effective monitoring of PRRSV infection.

Keywords

• porcine reproductive and respiratory syndrome virus
• screening of prrsv
• nucleocapsid n protein
• colloidal gold