Using lyophilized bovine pericardium and acellular ovine esophageal mucosa to repair cerebral dura mater defect in dogs

Abstract

This article assessed the ability of lyophilized bovine pericardium and acellular ovine esophageal mucosa with fibrin glue to repair induced cerebral dural defects in dogs. Eighteen adult male dogs were used and divided randomly into two groups. A square defect about (1x 1cm) was created in all animals in the dura mater. In the first group, the defect was closed with lyophilized bovine pericardium, while the defect was grafted with acellular ovine esophageal mucosa in the second group. The results were assessed by monitoring the clinical signs post-operation, the gross and histopathological changes, cerebrospinal fluid pressure with a level of glucose and protein, and total cell count of cerebral spinal fluid on the 15th, 30th, and 60th postoperative days. All animals were still alive without the appearance of any abnormal nervous signs after the operation. Grossly, in both groups, the craniectomy site was occluded completely, and no cerebrospinal fluid leaks were noticed postoperatively. Also, the dura mater of the host was connected with grafted subjects internally. The histopathological changes revealed connective tissue formation, infiltrations of inflammatory cells, and angiogenesis. The rapid formation of connective tissue and angiogenesis with little or no infiltration of inflammatory cells and total cell count were shown in the first rather than the second group. The cerebrospinal fluid pressure, glucose level, and protein appeared within the normal range in both groups study periods. It concluded; that both xenografted tissues with fibrin glue could be used to repair dural defects in dogs.

Keywords

• dura mater defect
• close of dural defect
• xenografted tissues