Nature Communications (Oct 2021)
PHF3 regulates neuronal gene expression through the Pol II CTD reader domain SPOC
- Lisa-Marie Appel,
- Vedran Franke,
- Melania Bruno,
- Irina Grishkovskaya,
- Aiste Kasiliauskaite,
- Tanja Kaufmann,
- Ursula E. Schoeberl,
- Martin G. Puchinger,
- Sebastian Kostrhon,
- Carmen Ebenwaldner,
- Marek Sebesta,
- Etienne Beltzung,
- Karl Mechtler,
- Gen Lin,
- Anna Vlasova,
- Martin Leeb,
- Rushad Pavri,
- Alexander Stark,
- Altuna Akalin,
- Richard Stefl,
- Carrie Bernecky,
- Kristina Djinovic-Carugo,
- Dea Slade
Affiliations
- Lisa-Marie Appel
- Department of Biochemistry and Cell Biology, Max Perutz Labs, University of Vienna, Vienna Biocenter (VBC)
- Vedran Franke
- The Berlin Institute for Medical Systems Biology, Max Delbrück Center
- Melania Bruno
- Department of Biochemistry and Cell Biology, Max Perutz Labs, University of Vienna, Vienna Biocenter (VBC)
- Irina Grishkovskaya
- Department of Structural and Computational Biology, Max Perutz Labs, University of Vienna, Vienna Biocenter (VBC)
- Aiste Kasiliauskaite
- CEITEC-Central European Institute of Technology, Masaryk University
- Tanja Kaufmann
- Department of Biochemistry and Cell Biology, Max Perutz Labs, University of Vienna, Vienna Biocenter (VBC)
- Ursula E. Schoeberl
- National Centre for Biomolecular Research, Faculty of Science, Masaryk University
- Martin G. Puchinger
- Department of Structural and Computational Biology, Max Perutz Labs, University of Vienna, Vienna Biocenter (VBC)
- Sebastian Kostrhon
- Department of Biochemistry and Cell Biology, Max Perutz Labs, University of Vienna, Vienna Biocenter (VBC)
- Carmen Ebenwaldner
- Department of Biochemistry and Cell Biology, Max Perutz Labs, University of Vienna, Vienna Biocenter (VBC)
- Marek Sebesta
- CEITEC-Central European Institute of Technology, Masaryk University
- Etienne Beltzung
- Department of Biochemistry and Cell Biology, Max Perutz Labs, University of Vienna, Vienna Biocenter (VBC)
- Karl Mechtler
- Research Institute of Molecular Pathology (IMP), Campus-Vienna-Biocenter 1, Vienna Biocenter (VBC)
- Gen Lin
- Research Institute of Molecular Pathology (IMP), Campus-Vienna-Biocenter 1, Vienna Biocenter (VBC)
- Anna Vlasova
- Research Institute of Molecular Pathology (IMP), Campus-Vienna-Biocenter 1, Vienna Biocenter (VBC)
- Martin Leeb
- Department of Microbiology, Immunobiology and Genetics, Max Perutz Labs, University of Vienna, Vienna Biocenter (VBC)
- Rushad Pavri
- Research Institute of Molecular Pathology (IMP), Campus-Vienna-Biocenter 1, Vienna Biocenter (VBC)
- Alexander Stark
- Research Institute of Molecular Pathology (IMP), Campus-Vienna-Biocenter 1, Vienna Biocenter (VBC)
- Altuna Akalin
- The Berlin Institute for Medical Systems Biology, Max Delbrück Center
- Richard Stefl
- CEITEC-Central European Institute of Technology, Masaryk University
- Carrie Bernecky
- Institute of Science and Technology Austria (IST Austria), Am Campus 1
- Kristina Djinovic-Carugo
- Department of Structural and Computational Biology, Max Perutz Labs, University of Vienna, Vienna Biocenter (VBC)
- Dea Slade
- Department of Biochemistry and Cell Biology, Max Perutz Labs, University of Vienna, Vienna Biocenter (VBC)
- DOI
- https://doi.org/10.1038/s41467-021-26360-2
- Journal volume & issue
-
Vol. 12,
no. 1
pp. 1 – 24
Abstract
Here the authors identify PHF3 SPOC domain as a reader of the phosphorylated RNA polymerase II (Pol II) C-terminal domain. They show that PHF3 clusters with Pol II complexes in cells, drives phase separation of Pol II in vitro, and regulates neuronal gene expression and neuronal differentiation.
