Generation of four iPSC lines from Neurofibromatosis Type 1 patients

Kiymet Bozaoglu; Wei Shern Lee; Kristina M. Haebich; Kathryn N. North; Jonathan M. Payne; Paul J. Lockhart

Stem Cell Research (Dec 2020)

Generation of four iPSC lines from Neurofibromatosis Type 1 patients

Kiymet Bozaoglu,
Wei Shern Lee,
Kristina M. Haebich,
Kathryn N. North,
Jonathan M. Payne,
Paul J. Lockhart

Affiliations

Kiymet Bozaoglu: Murdoch Children’s Research Institute, Parkville, Australia; Department of Paediatrics, University of Melbourne, Parkville, VIC, Australia; Corresponding author at: Murdoch Children's Research Institute, 50 Flemington Road Parkville, Victoria 3052, Australia.
Wei Shern Lee: Murdoch Children’s Research Institute, Parkville, Australia; Department of Paediatrics, University of Melbourne, Parkville, VIC, Australia
Kristina M. Haebich: Murdoch Children’s Research Institute, Parkville, Australia; Department of Paediatrics, University of Melbourne, Parkville, VIC, Australia
Kathryn N. North: Murdoch Children’s Research Institute, Parkville, Australia; Department of Paediatrics, University of Melbourne, Parkville, VIC, Australia
Jonathan M. Payne: Murdoch Children’s Research Institute, Parkville, Australia; Department of Paediatrics, University of Melbourne, Parkville, VIC, Australia; Royal Children’s Hospital, Parkville, VIC, Australia
Paul J. Lockhart: Murdoch Children’s Research Institute, Parkville, Australia; Department of Paediatrics, University of Melbourne, Parkville, VIC, Australia

Journal volume & issue: Vol. 49
p. 102013

Abstract

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We describe the generation and characterisation of four human induced pluripotent stem cell (iPSC) lines from peripheral blood mononuclear cells (PBMC) from individuals with neurofibromatosis type (NF1). PBMC reprogramming was performed using a non-integrative Sendai virus containing the reprogramming factors OCT4, SOX2, MYC and KLF4. All iPSC lines exhibited a normal karyotype, and pluripotency was validated by flow cytometry (EPCAM, TRA-1-81, SSEA1 and CD9) and immunofluorescence (OCT4 and Nanog). Differentiation of the cells into the three embryonic germ layers was confirmed using immunofluorescence. These iPSC lines are a valuable pre-clinical resource to study the molecular mechanisms underlying NF1.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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