Generation and characterization of a human induced pluripotent stem cell line UOWi005-A from dermal fibroblasts derived from a CCNFS621G familial amyotrophic lateral sclerosis patient using mRNA reprogramming

Monique Bax; Rachelle Balez; Sonia Sanz Muñoz; Dzung Do-Ha; Claire H. Stevens; Tracey Berg; Mauricio C. Cabral-da-Silva; Martin Engel; Garth Nicholson; Shu Yang; Ian P. Blair; Lezanne Ooi

Stem Cell Research (Oct 2019)

Generation and characterization of a human induced pluripotent stem cell line UOWi005-A from dermal fibroblasts derived from a CCNFS621G familial amyotrophic lateral sclerosis patient using mRNA reprogramming

Monique Bax,
Rachelle Balez,
Sonia Sanz Muñoz,
Dzung Do-Ha,
Claire H. Stevens,
Tracey Berg,
Mauricio C. Cabral-da-Silva,
Martin Engel,
Garth Nicholson,
Shu Yang,
Ian P. Blair,
Lezanne Ooi

Affiliations

Monique Bax: Illawarra Health and Medical Research Institute, Wollongong, NSW 2522, Australia; School of Chemistry and Molecular Bioscience, University of Wollongong, Wollongong, NSW 2522, Australia
Rachelle Balez: Illawarra Health and Medical Research Institute, Wollongong, NSW 2522, Australia; School of Chemistry and Molecular Bioscience, University of Wollongong, Wollongong, NSW 2522, Australia
Sonia Sanz Muñoz: Illawarra Health and Medical Research Institute, Wollongong, NSW 2522, Australia; School of Chemistry and Molecular Bioscience, University of Wollongong, Wollongong, NSW 2522, Australia
Dzung Do-Ha: Illawarra Health and Medical Research Institute, Wollongong, NSW 2522, Australia; School of Chemistry and Molecular Bioscience, University of Wollongong, Wollongong, NSW 2522, Australia
Claire H. Stevens: Illawarra Health and Medical Research Institute, Wollongong, NSW 2522, Australia; School of Chemistry and Molecular Bioscience, University of Wollongong, Wollongong, NSW 2522, Australia
Tracey Berg: Illawarra Health and Medical Research Institute, Wollongong, NSW 2522, Australia; School of Chemistry and Molecular Bioscience, University of Wollongong, Wollongong, NSW 2522, Australia
Mauricio C. Cabral-da-Silva: Illawarra Health and Medical Research Institute, Wollongong, NSW 2522, Australia; School of Chemistry and Molecular Bioscience, University of Wollongong, Wollongong, NSW 2522, Australia
Martin Engel: Illawarra Health and Medical Research Institute, Wollongong, NSW 2522, Australia; School of Chemistry and Molecular Bioscience, University of Wollongong, Wollongong, NSW 2522, Australia
Garth Nicholson: University of Sydney ANZAC Research Institute, Concord Repatriation General Hospital, Sydney, Australia
Shu Yang: Macquarie University Centre for Motor Neuron Disease Research, Macquarie University Faculty of Medicine and Health Sciences, NSW 2109, Australia
Ian P. Blair: Macquarie University Centre for Motor Neuron Disease Research, Macquarie University Faculty of Medicine and Health Sciences, NSW 2109, Australia
Lezanne Ooi: Illawarra Health and Medical Research Institute, Wollongong, NSW 2522, Australia; School of Chemistry and Molecular Bioscience, University of Wollongong, Wollongong, NSW 2522, Australia; Corresponding author at: Illawarra Health and Medical Research Institute, Wollongong, NSW 2522, Australia.

Journal volume & issue: Vol. 40

Abstract

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Dermal fibroblasts from a 59 year old male patient with amyotrophic lateral sclerosis (symptomatic at the time of collection), attributed to a mutation in the cyclin F gene (CCNFS621G), were reprogrammed using mRNA and microRNA-delivered OSKM factors to induced pluripotent stem cells (iPSCs). The generated iPSCs were confirmed pluripotent, expressing typical pluripotency markers and were capable of three germ layer differentiation. This is the first reported reprogramming of cells with a mutation in the cyclin F gene, and represents a novel resource for the study of amyotrophic lateral sclerosis.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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