Materials (May 2020)

Comparative Surface Morphology, Chemical Composition, and Cytocompatibility of Bio-C Repair, Biodentine, and ProRoot MTA on hDPCs

  • James Ghilotti,
  • José Luis Sanz,
  • Sergio López-García,
  • Julia Guerrero-Gironés,
  • María P. Pecci-Lloret,
  • Adrián Lozano,
  • Carmen Llena,
  • Francisco Javier Rodríguez-Lozano,
  • Leopoldo Forner,
  • Gianrico Spagnuolo

DOI
https://doi.org/10.3390/ma13092189
Journal volume & issue
Vol. 13, no. 9
p. 2189

Abstract

Read online

Biocompatibility is an essential property for any vital pulp material that may interact with the dental pulp tissues. Accordingly, this study aimed to compare the chemical composition and ultrastructural morphology of Biodentine (Septodont, Saint Maur-des-Fosses, France), ProRoot MTA (Dentsply Tulsa Dental Specialties, Johnson City, TN, USA), and Bio-C Repair (Angelus, Londrina, PR, Brazil), as well as their biological effects on human dental pulp cells. Chemical element characterization of the materials was undertaken using scanning electron microscopy and energy dispersive X-ray analysis (SEM-EDX). The cytotoxicity was assessed by analyzing the cell viability (MTT assay), cell morphology (immunofluorescence assay), and cell attachment (flow cytometry assay). The results were statistically analyzed using ANOVA and Tukey’s test (p p < 0.001). Based on the evidence derived from an in vitro cellular study, it was concluded that Bio-C Repair showed excellent cytocompatibility that was similar to Biodentine and ProRoot MTA.

Keywords