mBio (Jun 2021)

Shared and Unique Evolutionary Trajectories to Ciprofloxacin Resistance in Gram-Negative Bacterial Pathogens

  • Jaime E. Zlamal,
  • Semen A. Leyn,
  • Mallika Iyer,
  • Marinela L. Elane,
  • Nicholas A. Wong,
  • James W. Wamsley,
  • Maarten Vercruysse,
  • Fernando Garcia-Alcalde,
  • Andrei L. Osterman

DOI
https://doi.org/10.1128/mBio.00987-21
Journal volume & issue
Vol. 12, no. 3

Abstract

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The challenge of spreading antibiotic resistance calls for systematic efforts to develop more “irresistible” drugs based on a deeper understanding of dynamics and mechanisms of antibiotic resistance acquisition. To address this challenge, we have established a comparative resistomics approach which combines experimental evolution in a continuous-culturing device, the morbidostat, with ultradeep sequencing of evolving microbial populations to identify evolutionary trajectories (mutations and genome rearrangements) leading to antibiotic resistance over a range of target pathogens.