OncoTargets and Therapy (Dec 2019)

Long Non-Coding RNA HULC Promotes the Development of Breast Cancer Through Regulating LYPD1 Expression by Sponging miR-6754-5p

  • Wang N,
  • Zhong C,
  • Fu M,
  • Li L,
  • Wang F,
  • Lv P,
  • Zhu M,
  • Xiong Y,
  • Mi H,
  • Gu Y

Journal volume & issue
Vol. Volume 12
pp. 10671 – 10679

Abstract

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Nan Wang,1 Chaochao Zhong,2 Mingti Fu,3 Lin Li,1 Fang Wang,1 Pengwei Lv,1 Mingzhi Zhu,1 Youyi Xiong,1 Hailong Mi,1 Yuanting Gu1 1Department of Breast Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan, People’s Republic of China; 2Department of Emergency, The First People’s Hospital of Zhengzhou, Zhengzhou 450052, Henan, People’s Republic of China; 3Department of General Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan, People’s Republic of ChinaCorrespondence: Yuanting GuDepartment of Breast Surgery, The First Affiliated Hospital of Zhengzhou University, Erqi District, Zhengzhou 450052, People’s Republic of ChinaEmail [email protected]: Long non-coding RNAs (lncRNAs) were found to regulate many biological processes including cancer development, immunology and other diseases. LncRNA HULC was found to be oncogenes in many cancer progression. However, the role of HULC in the regulation of breast cancer remains unclear.Methods: The expression of HULC and miR-6754-5p was examined by RT-PCR. Through knockdown of HULC, we found that the proliferation abilities coupled with migration and invasion abilities were significantly decreased. And also, we verified that overexpression of miR-6754-5p significantly decreased the proliferation ability of breast cancer cells.Results: In this study, we found that lncRNA HULC was overexpressed in breast cancer tissues and cell lines compared to normal healthy breast tissues and normal breast cell line. Moreover, the high expression of HULC was associated with metastasis and malignancy of breast cancers. Mechanically, we found that HULC can bind to miR-6754-5p directly through complementary base pairing. Furthermore, we found that HULC regulates the expression of LYPD1 through sponging miR-6754-5p. Moreover, overexpression of LYPD1 can rescue the migration and invasion abilities of breast cancer cells decreased by knockdown of HULC or overexpression of miR-6754-5p.Conclusion: Our study showed the role of HULC in promoting breast cancer development and explained the detailed molecular mechanisms.Keywords: lncRNA HULC, breast cancer development, miR-6754-5p, LYPD1

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