Comparative data analsysis of two multi-drug resistant homoserine lactone and rhamnolipid producing Pseudomonas aeruginosa from diabetic foot infected patient
Prakhar Srivastava,
Sankaranarayanan Gomathinayagam,
Nalini Easwaran,
Gowri Sankar,
E. Padmavathi,
Manoharan Shankar,
Kodiveri M. Gothandam,
Karthikeyan Sivashanmugam
Affiliations
Prakhar Srivastava
School of Bio Sciences and Technology, Vellore Institute of Technology, Vellore 632014 India
Sankaranarayanan Gomathinayagam
School of Bio Sciences and Technology, Vellore Institute of Technology, Vellore 632014 India
Nalini Easwaran
School of Bio Sciences and Technology, Vellore Institute of Technology, Vellore 632014 India
Gowri Sankar
School of Bio Sciences and Technology, Vellore Institute of Technology, Vellore 632014 India
E. Padmavathi
School of Bio Sciences and Technology, Vellore Institute of Technology, Vellore 632014 India
Manoharan Shankar
School of Bio Sciences and Technology, Vellore Institute of Technology, Vellore 632014 India
Kodiveri M. Gothandam
School of Bio Sciences and Technology, Vellore Institute of Technology, Vellore 632014 India
Karthikeyan Sivashanmugam
Corresponding author.; School of Bio Sciences and Technology, Vellore Institute of Technology, Vellore 632014 India
Pseudomonas aeruginosa generally forms strong biofilm during chronic condition of wound. The whole mechanism of biofilm formation works in tandem with quorum sensing circuit of the organism in order to produce virulence. Here we report the draft genome sequence of two diabetic foot ulcer Pseudomonas aeruginosa isolates (VIT PC 7 and VIT PC 9) displaying homoserine lactone, rhamnolipid producing, biofilm phenotype and antibiotic resistance genes related to carbapenem, aminoglycoside, beta- lactamase and tetracycline resistance. The whole genome sequencing library was prepared according to the Oxford Nanopore's SQK-LSK108 kit protocol on Oxford Nanopore's Minion platform. The 7.1 Mb and 6.3-Mb draft genome sequence with GC content of 65.8% and 66.4% respectively provides insight into their resistance mechanism and virulence factors.
