Protocol to develop a synthetic biology toolkit for the non-model bacterium R. palustris

Abstract

Summary: Numerous biology tools are developed to work for model organisms, which, however, do not work effectively in non-model organisms. Here, we present a protocol for developing a synthetic biology toolkit for Rhodopseudomonas palustris CGA009, a non-model bacterium with unique metabolic properties. We describe steps for introducing and characterizing biological devices in non-model bacteria, such as the utilization of fluorescence markers and RT-qPCR. This protocol may also be applicable for other non-model organisms.For complete details on the use and execution of this protocol, please refer to Immethun et al..1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

Keywords

• Genetics
• Metabolism
• Microbiology
• Molecular Biology
• Gene Expression
• Molecular/Chemical Probes