CYTOGENETIC DETECTION OF INFECTIOUS AGENTS IN IXODID TICKS

Abstract

Aim: To test whether the cytogenetic techniques can be used for detection of infectious agents in ixodid ticks. Materials and Methods: Suspensions of ixodid ticks were tested for encephalitis virus (TBEV) and pathogenic Borrelia (PB) using enzymelinked immunosorbent assay and then added to the lymphocytes isolated from donor peripheral blood. Results: Suspensions of ixodid ticks with either TBEV or ITBB induced clastogenic and aneugenic effects, with the synergistic action of these two agents. However, one-third of samples negative for both TBEV and PB did still provoke mutagenic effects. To test whether other infectious agents may be responsible for this, we conducted polymerase chain reaction, with the successful identification of West Nile Fever virus (WNFV). Conclusion: Cytogenetic techniques can be used for the detection of TBEV, ITBB, or WNFV in ixodid ticks.

Keywords

• цитогенетический метод
• иксодовые клещи
• вирус клещевого энцефалита
• патогенные borrelia
• клещевые иксодовые боррелиозы
• вирус лихорадки западного нила
• cytogenetics
• ixodid ticks
• tick-borne encephalitis virus
• pathogenic borrelia
• ixodes tick-borne borreliosis
• west nile fever virus