Development of the Electric Equivalent Model for the Cytoplasmic Microinjection of Small Adherent Cells

Abstract

A novel approach utilizing current feedback for the cytoplasmic microinjection of biological cells is proposed. In order to realize the cytoplasmic microinjection on small adherent cells (diameter < 30 μm and thickness < 10 μm), an electrical model is built and analyzed according to the electrochemical properties of target cells. In this study, we have verified the effectiveness of the current measurement for monitoring the injection process and the study of ion channel activities for verifying the cell viability of the cells after the microinjection.

Keywords

• cell cytoplasmic microinjection
• small adherent cells
• equivalent-circuit model