Effects of Heat Stress and Exogenous Salicylic Acid on Secondary Metabolites Biosynthesis in <i>Pleurotus ostreatus</i> (Jacq.) P. Kumm

Yanru Hu; Qianqian Chai; Yue Wang; Yujie Chen; Haozhe Dong; Jinwen Shen; Yuancheng Qi; Haiyou Yu; Fengqin Wang; Qing Wen

doi:10.3390/life12060915

Life (Jun 2022)

Effects of Heat Stress and Exogenous Salicylic Acid on Secondary Metabolites Biosynthesis in <i>Pleurotus ostreatus</i> (Jacq.) P. Kumm

Yanru Hu,
Qianqian Chai,
Yue Wang,
Yujie Chen,
Haozhe Dong,
Jinwen Shen,
Yuancheng Qi,
Haiyou Yu,
Fengqin Wang,
Qing Wen

Affiliations

Yanru Hu: Key Laboratory of Agricultural Microbial Enzyme Engineering, Ministry of Agriculture, Rural Department, College of Life Sciences, Henan Agricultural University, Zhengzhou 450002, China
Qianqian Chai: Key Laboratory of Agricultural Microbial Enzyme Engineering, Ministry of Agriculture, Rural Department, College of Life Sciences, Henan Agricultural University, Zhengzhou 450002, China
Yue Wang: Key Laboratory of Agricultural Microbial Enzyme Engineering, Ministry of Agriculture, Rural Department, College of Life Sciences, Henan Agricultural University, Zhengzhou 450002, China
Yujie Chen: Key Laboratory of Agricultural Microbial Enzyme Engineering, Ministry of Agriculture, Rural Department, College of Life Sciences, Henan Agricultural University, Zhengzhou 450002, China
Haozhe Dong: Key Laboratory of Agricultural Microbial Enzyme Engineering, Ministry of Agriculture, Rural Department, College of Life Sciences, Henan Agricultural University, Zhengzhou 450002, China
Jinwen Shen: Key Laboratory of Agricultural Microbial Enzyme Engineering, Ministry of Agriculture, Rural Department, College of Life Sciences, Henan Agricultural University, Zhengzhou 450002, China
Yuancheng Qi: Key Laboratory of Agricultural Microbial Enzyme Engineering, Ministry of Agriculture, Rural Department, College of Life Sciences, Henan Agricultural University, Zhengzhou 450002, China
Haiyou Yu: College of Food and Bioengineering, Henan University of Animal Husbandry and Economy, Zhengzhou 450002, China
Fengqin Wang: Key Laboratory of Agricultural Microbial Enzyme Engineering, Ministry of Agriculture, Rural Department, College of Life Sciences, Henan Agricultural University, Zhengzhou 450002, China
Qing Wen: Key Laboratory of Agricultural Microbial Enzyme Engineering, Ministry of Agriculture, Rural Department, College of Life Sciences, Henan Agricultural University, Zhengzhou 450002, China

DOI: https://doi.org/10.3390/life12060915
Journal volume & issue: Vol. 12, no. 6
p. 915

Abstract

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Pleurotus ostreatus (Jacq.) P. Kumm has high medicinal value, but few studies exist on regulating secondary metabolite biosynthesis. Environmental factors play a substantial role in the accumulation of microbial secondary metabolites. In this study, the effects of heat stress (24 h) and salicylic acid (0.05 mmol/L) treatment on the secondary metabolism of P. ostreatus were analyzed by metabolome, transcriptome, and gene differential expression analysis. Metabolome and transcriptome analyses showed that salicylic acid significantly increased the accumulation of antibiotics and polyketones, while heat stress increased the accumulation of flavonoids, polyketones, terpenoids, and polysaccharides. The content and the biosynthetic genes expression of heparin were markedly increased by heat stress, and the former was increased by 4565.54-fold. This study provides a reference for future studies on secondary metabolite accumulation in edible fungi.

Published in Life

ISSN: 2075-1729 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science
Website: http://www.mdpi.com/journal/life

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