Revista Ciencias de la Salud (Dec 2005)
Analysis of Urine Samples for the Molecular Detection of Infectious Diseases. Application to the Identification of Human Cytomegalovirus
Abstract
Molecular biology methods like Polimerase ChainReaction (PCR) has been used for diagnosis ofinfectious diseases. Until today, the identificationmethods are based mainly on cultures and serologydue to their sensibility and specificity, but they areexpensive and time consuming. Urine samplesconstitute an alternative, noninvasive, method ofobtaining DNA for the accomplishment of molecularBiology analysis. Methodology: implementationof a strategy to obtain DNA from urinesamples. Samples were taken from children in daycarecenters, to document the presence of inhibitors,PCR amplification of genes of human Cytomegalovirus(HCMV) was done. Results: In 27.1%of the analyzed samples, specific amplification forHCMV was demonstrated. No viral significantdifferences were found in the three layers, althoughit was present in the bands. Conclusion: The inhibitorabsence was verified using PCR by amplificatingthe gene of the B-globine. A molecular methodologyfor the HCMV identification was standardized,which can be applied in prenatal diagnosis of congenitalinfection.
