Clinical Evaluation of QuickNavi<sup>TM</sup>-Ebola in the 2018 Outbreak of Ebola Virus Disease in the Democratic Republic of the Congo

Sheila Makiala; Daniel Mukadi; Anja De Weggheleire; Shino Muramatsu; Daisuke Kato; Koichi Inano; Fumio Gondaira; Masahiro Kajihara; Reiko Yoshida; Katendi Changula; Aaron Mweene; Placide Mbala-Kingebeni; Jean-Jacques Muyembe-Tamfum; Justin Masumu; Steve Ahuka; Ayato Takada

doi:10.3390/v11070589

Viruses (Jun 2019)

Clinical Evaluation of QuickNavi<sup>TM</sup>-Ebola in the 2018 Outbreak of Ebola Virus Disease in the Democratic Republic of the Congo

Sheila Makiala,
Daniel Mukadi,
Anja De Weggheleire,
Shino Muramatsu,
Daisuke Kato,
Koichi Inano,
Fumio Gondaira,
Masahiro Kajihara,
Reiko Yoshida,
Katendi Changula,
Aaron Mweene,
Placide Mbala-Kingebeni,
Jean-Jacques Muyembe-Tamfum,
Justin Masumu,
Steve Ahuka,
Ayato Takada

Affiliations

Sheila Makiala: Institut National de Recherche Biomédicale, Avenue de la Démocratie, Kinshasa/Gombe-P.O. Box 1197, Kinshasa I, Democratic Republic of the Congo
Daniel Mukadi: Institut National de Recherche Biomédicale, Avenue de la Démocratie, Kinshasa/Gombe-P.O. Box 1197, Kinshasa I, Democratic Republic of the Congo
Anja De Weggheleire: Department of Clinical Sciences, Institute of Tropical Medicine, Antwerp, Belgium
Shino Muramatsu: Denka Seiken Co., Ltd., 1359-1, Kagamida, Kigoshi, Gosen, Niigata 959-1695, Japan
Daisuke Kato: Denka Seiken Co., Ltd., 1359-1, Kagamida, Kigoshi, Gosen, Niigata 959-1695, Japan
Koichi Inano: Denka Seiken Co., Ltd., 1359-1, Kagamida, Kigoshi, Gosen, Niigata 959-1695, Japan
Fumio Gondaira: Denka Seiken Co., Ltd., 1359-1, Kagamida, Kigoshi, Gosen, Niigata 959-1695, Japan
Masahiro Kajihara: Division of Global Epidemiology, Research Center for Zoonosis Control, Hokkaido University, Kita-20, Nishi-10, Kita-ku, Sapporo 001-0020, Japan
Reiko Yoshida: Division of Global Epidemiology, Research Center for Zoonosis Control, Hokkaido University, Kita-20, Nishi-10, Kita-ku, Sapporo 001-0020, Japan
Katendi Changula: School of Veterinary Medicine, the University of Zambia, Great East Road Campus, Lusaka, Zambia
Aaron Mweene: School of Veterinary Medicine, the University of Zambia, Great East Road Campus, Lusaka, Zambia
Placide Mbala-Kingebeni: Institut National de Recherche Biomédicale, Avenue de la Démocratie, Kinshasa/Gombe-P.O. Box 1197, Kinshasa I, Democratic Republic of the Congo
Jean-Jacques Muyembe-Tamfum: Institut National de Recherche Biomédicale, Avenue de la Démocratie, Kinshasa/Gombe-P.O. Box 1197, Kinshasa I, Democratic Republic of the Congo
Justin Masumu: Institut National de Recherche Biomédicale, Avenue de la Démocratie, Kinshasa/Gombe-P.O. Box 1197, Kinshasa I, Democratic Republic of the Congo
Steve Ahuka: Institut National de Recherche Biomédicale, Avenue de la Démocratie, Kinshasa/Gombe-P.O. Box 1197, Kinshasa I, Democratic Republic of the Congo
Ayato Takada: Division of Global Epidemiology, Research Center for Zoonosis Control, Hokkaido University, Kita-20, Nishi-10, Kita-ku, Sapporo 001-0020, Japan

DOI: https://doi.org/10.3390/v11070589
Journal volume & issue: Vol. 11, no. 7
p. 589

Abstract

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The recent large outbreaks of Ebola virus disease (EVD) in West Africa and the Democratic Republic of the Congo (DRC) have highlighted the need for rapid diagnostic tests to control this disease. In this study, we clinically evaluated a previously developed immunochromatography-based kit, QuickNaviTM-Ebola. During the 2018 outbreaks in DRC, 928 blood samples from EVD-suspected cases were tested with QuickNaviTM-Ebola and the WHO-approved GeneXpert. The sensitivity and specificity of QuickNaviTM-Ebola, estimated by comparing it to GeneXpert-confirmed cases, were 85% (68/80) and 99.8% (846/848), respectively. These results indicate the practical reliability of QuickNaviTM-Ebola for point-of-care diagnosis of EVD.

Published in Viruses

ISSN: 1999-4915 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Microbiology
Website: http://www.mdpi.com/journal/viruses

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