OncoTargets and Therapy (Aug 2019)
LncRNA ELF3-AS1 is involved in the regulation of oral squamous cell carcinoma cell proliferation by reprogramming glucose metabolism
Abstract
Hongxing Chu,* Zhengqiang Li,* Zekun Gan, Zinan Yang, Zhenzhen Wu, Mingdeng RongDepartment of Periodontics and Implantology, Stomatological Hospital, Southern Medical University (Guangdong Provincial Stomatological Hospital), Guangzhou City, Guangdong Province 510280, People’s Republic of ChinaCorrespondence: Mingdeng RongDepartment of Periodontics and Implantology, Stomatological Hospital, Southern Medical University (Guangdong Provincial Stomatological Hospital), No. 366 Jiangnan Avenue South, Guangzhou City, Guangdong Province 510280, People’s Republic of ChinaTel +86 208 442 7034Email [email protected]*These authors contributed equally to this workPurpose: The present study aims to investigate the role of ELF3-AS1 in oral squamous cell carcinoma (OSCC).Patients and methods: A total of 112 patients with OSCC were admitted in Guangdong Provincial Stomatological Hospital from March 2016 to March 2019. RT-qPCR, cells and transient transfections, cell proliferation rate measurements and Western blots were carried out to analyze the samples.Results: In the present study, we showed that ELF3-AS1 and glucose transporter 1 (GLUT1) were both upregulated in OSCC tissues, and those two factors were positively correlated. In OSCC cells, ELF3-AS1 overexpression resulted in upregulation, while ELF3-AS1 siRNA silencing caused downregulated expression of GLUT1 and glucose uptake. ELF3-AS1 and GLUT1 overexpression resulted in increased rate of OSCC cells, while ELF3-AS1 and GLUT1 siRNA silencing resulted in decreased proliferation rate of OSCC cells. In addition, GLUT1 siRNA silencing attenuated the effects of ELF3-AS1 overexpression.Conclusion: Therefore, ELF3-AS1 promotes the proliferation of OSCC cells by reprogramming glucose metabolism.Keywords: oral squamous cell carcinoma, lncRNA ELF3-AS1, GLUT1, glucose uptake
