In vitro study of curcumin calcium carbonate phosphate nanoparticles (Curcumin-NPs) impacts on the meriz goat’s coccidian oocysts

Abstract

Nanoparticles biosynthesis has an essential and increased role in delivering medical compounds. Calcium carbonate phosphate nanoparticles (CaCO3-NPs) were prepared as a stabilized amorphous and incorporated with herbal curcumin extract as an anticoccidial agent in vitro. CaCO3 - NPs were tested against local meriz goat coccidian oocysts. Concentrations were used 2, 4, 8, 16, 30 and 50 mg/ml shows oocysticidal effects and sporocystidal effects at concentration of 100, 200, 400, 800 and 1000 µg. Sporulation inhibition assay was used for 24 and 48 hours. Results of significant oocysticidal effect were seen to inhibit in the concentration of 30 - 50 mg/ml and able to inhibit the sporulation of meriz coccidian parasite oocysts at a rate of 92.54±3.51%. The sporocysticidal effect was also significant with a curcumin nanoparticles concentration of 400-1000 µg/ml with a rate of 98.1±2.11%. The stability of prepared curcumin nanoparticles was examined against various pH levels 4.01, 7, and 9.21 at multiple temperatures 4, 25, 60, and 100°C. Investigation after 1, 6, 12, and 24 hours of treatment occurs according to various treatments. Stability was assessed by spectrophotometric indicated significant reductions for pH 4 and 9 after one hour of treatment and at the temperature of 60°C and 100°C after 12-24 hours of treatment. These results reflect promising hopes of exploiting CaCO3 curcumin nanoparticles to eradicate coccidiosis as they are composed of and prepared from natural substances.

Keywords

• nanoparticles
• curcumin
• caco3
• coccidia
• meriz goats