Qualitative phytochemicals and pharmacological properties analysis of methanol extract of Tabernaemontana pauciflora leaves

Abstract

The methanol extract of the leaves of Tabernaemontana pauciflora (METP) was screened to investigate the semi-qualitative phytochemical, in vivo anti-depressant, anxiolytic and in vitro thrombolytic activities. The in vivo antidepressant activity of METP was examined by Force swimming test (FST) and Tail suspension test (TST) whereas anxiolytic activity was examined by using Hole-board test (HBT) and Elevated plus maze test (EPM) on mice model. The in vitro thrombolytic model was used to check the clot lysis effect of METP. Additionally, semi-qualitative phytochemical assay was performed through in vitro test. In the antidepressant activity, METP was exhibited significant value of FST (45.64% & 58.31%) and TST (38.67% & 63.62%) whereas the notable anxiolytic effect was observed in EPM (67.67% & 56.06%) and HBT (84) in dose dependent manner which compared with control and standard. In in vitro thrombolytic assessment of METP exposed 22.66% inhibition of clot. The current results proposed that METP might be effective in neuropharmacological and thrombolytic response due to the presence of alkaloids, tannins and flavonoids, which required further studies to investigate the mechanism. [ J Adv Biotechnol Exp Ther 2021; 4(1.000): 9-16]

Keywords

• tabernaemontana pauciflora
• anxiolytic
• anti-depressant
• thrombolytic