Frontiers in Microbiology (Jul 2024)
Identification of potential nucleomodulins of Mycoplasma bovis by direct biotinylation and proximity-based biotinylation approaches
- Doukun Lu,
- Doukun Lu,
- Doukun Lu,
- Doukun Lu,
- Doukun Lu,
- Jiongxi Chen,
- Jiongxi Chen,
- Jiongxi Chen,
- Jiongxi Chen,
- Jiongxi Chen,
- Menghan Zhang,
- Menghan Zhang,
- Menghan Zhang,
- Menghan Zhang,
- Menghan Zhang,
- Yingjie Fu,
- Yingjie Fu,
- Yingjie Fu,
- Yingjie Fu,
- Yingjie Fu,
- Abdul Raheem,
- Abdul Raheem,
- Abdul Raheem,
- Abdul Raheem,
- Abdul Raheem,
- Yingyu Chen,
- Yingyu Chen,
- Yingyu Chen,
- Yingyu Chen,
- Yingyu Chen,
- Xi Chen,
- Xi Chen,
- Xi Chen,
- Xi Chen,
- Xi Chen,
- Changmin Hu,
- Changmin Hu,
- Changmin Hu,
- Changmin Hu,
- Changmin Hu,
- Jianguo Chen,
- Jianguo Chen,
- Jianguo Chen,
- Jianguo Chen,
- Jianguo Chen,
- Elise Schieck,
- Gang Zhao,
- Aizhen Guo,
- Aizhen Guo,
- Aizhen Guo,
- Aizhen Guo,
- Aizhen Guo
Affiliations
- Doukun Lu
- National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China
- Doukun Lu
- Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, China
- Doukun Lu
- College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China
- Doukun Lu
- Hubei International Scientific and Technological Cooperation Base of Veterinary Epidemiology, Huazhong Agricultural University, Wuhan, China
- Doukun Lu
- International Research Center for Animal Disease, Ministry of Science and Technology, Huazhong Agricultural University, Wuhan, China
- Jiongxi Chen
- National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China
- Jiongxi Chen
- Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, China
- Jiongxi Chen
- College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China
- Jiongxi Chen
- Hubei International Scientific and Technological Cooperation Base of Veterinary Epidemiology, Huazhong Agricultural University, Wuhan, China
- Jiongxi Chen
- International Research Center for Animal Disease, Ministry of Science and Technology, Huazhong Agricultural University, Wuhan, China
- Menghan Zhang
- National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China
- Menghan Zhang
- Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, China
- Menghan Zhang
- College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China
- Menghan Zhang
- Hubei International Scientific and Technological Cooperation Base of Veterinary Epidemiology, Huazhong Agricultural University, Wuhan, China
- Menghan Zhang
- International Research Center for Animal Disease, Ministry of Science and Technology, Huazhong Agricultural University, Wuhan, China
- Yingjie Fu
- National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China
- Yingjie Fu
- Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, China
- Yingjie Fu
- College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China
- Yingjie Fu
- Hubei International Scientific and Technological Cooperation Base of Veterinary Epidemiology, Huazhong Agricultural University, Wuhan, China
- Yingjie Fu
- International Research Center for Animal Disease, Ministry of Science and Technology, Huazhong Agricultural University, Wuhan, China
- Abdul Raheem
- National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China
- Abdul Raheem
- Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, China
- Abdul Raheem
- College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China
- Abdul Raheem
- Hubei International Scientific and Technological Cooperation Base of Veterinary Epidemiology, Huazhong Agricultural University, Wuhan, China
- Abdul Raheem
- International Research Center for Animal Disease, Ministry of Science and Technology, Huazhong Agricultural University, Wuhan, China
- Yingyu Chen
- National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China
- Yingyu Chen
- Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, China
- Yingyu Chen
- College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China
- Yingyu Chen
- Hubei International Scientific and Technological Cooperation Base of Veterinary Epidemiology, Huazhong Agricultural University, Wuhan, China
- Yingyu Chen
- International Research Center for Animal Disease, Ministry of Science and Technology, Huazhong Agricultural University, Wuhan, China
- Xi Chen
- National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China
- Xi Chen
- Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, China
- Xi Chen
- College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China
- Xi Chen
- Hubei International Scientific and Technological Cooperation Base of Veterinary Epidemiology, Huazhong Agricultural University, Wuhan, China
- Xi Chen
- International Research Center for Animal Disease, Ministry of Science and Technology, Huazhong Agricultural University, Wuhan, China
- Changmin Hu
- National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China
- Changmin Hu
- Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, China
- Changmin Hu
- College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China
- Changmin Hu
- Hubei International Scientific and Technological Cooperation Base of Veterinary Epidemiology, Huazhong Agricultural University, Wuhan, China
- Changmin Hu
- International Research Center for Animal Disease, Ministry of Science and Technology, Huazhong Agricultural University, Wuhan, China
- Jianguo Chen
- National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China
- Jianguo Chen
- Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, China
- Jianguo Chen
- College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China
- Jianguo Chen
- Hubei International Scientific and Technological Cooperation Base of Veterinary Epidemiology, Huazhong Agricultural University, Wuhan, China
- Jianguo Chen
- International Research Center for Animal Disease, Ministry of Science and Technology, Huazhong Agricultural University, Wuhan, China
- Elise Schieck
- International Livestock Research Institute, Nairobi, Kenya
- Gang Zhao
- Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the Western China, School of Life Sciences, Ningxia University, Yinchuan, China
- Aizhen Guo
- National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China
- Aizhen Guo
- Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, China
- Aizhen Guo
- College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China
- Aizhen Guo
- Hubei International Scientific and Technological Cooperation Base of Veterinary Epidemiology, Huazhong Agricultural University, Wuhan, China
- Aizhen Guo
- International Research Center for Animal Disease, Ministry of Science and Technology, Huazhong Agricultural University, Wuhan, China
- DOI
- https://doi.org/10.3389/fmicb.2024.1421585
- Journal volume & issue
-
Vol. 15
Abstract
Mycoplasma bovis (M. bovis) is a significant bovine pathogen associated with various diseases, including bovine bronchopneumonia and mastitis resulting in substantial economic losses within the livestock industry. However, the development of effective control measures for M. bovis is hindered by a limited understanding of its virulence factors and pathogenesis. Nucleomodulins are newly identified secreted proteins of bacteria that internalize the host nuclei to regulate host cell gene expression and serve as critical virulence factors. Although recent reports have initiated exploration of mycoplasma nucleomodulins, the efficiency of conventional techniques for identification is very limited. Therefore, this study aimed to establish high-throughput methods to identify novel nucleomodulins of M. bovis. Using a direct biotinylation (DB) approach, a total of 289 proteins were identified including 66 high abundant proteins. In parallel, the use of proximity-based biotinylation (PBB), identified 28 proteins. Finally, seven nucleomodulins were verified to be nuclear by transfecting the bovine macrophage cell line BoMac with the plasmids encoding EGFP-fused proteins and observed with Opera Phenix, including the known nucleomodulin MbovP475 and six novel nucleomodulins. The novel nucleomodulins were four ribosomal proteins (MbovP599, MbovP678, MbovP710, and MbovP712), one transposase (MbovP790), and one conserved hypothetical protein (MbovP513). Among them, one unique nucleomodulin MbovP475 was identified with DB, two unique nucleomodulins (MbovP513 and MbovP710) with PBB, and four nucleomodulins by both. Overall, these findings established a foundation for further research on M. bovis nucleomodulin-host interactions for identification of new virulence factors.
Keywords
