Fast and Efficient Mouse Pluripotency Reprogramming Using a Chemically-Defined Medium

Abstract

The reprogramming of somatic cells to obtain induced pluripotent stem cells (iPSCs) is an important biological and medical breakthrough, providing important applications for fields such as regenerative medicine and disease modeling. However, this promising technology is damped due to its low efficiency and slow kinetics. Therefore, we generated a practical workflow to rapidly and efficiently induce iPSCs from mouse embryonic fibroblasts (MEFs) using iCD1 (iPS chemically-defined medium 1). This protocol can easily be implemented in a standard cell culture laboratory and be applied to cell fate research.

Keywords

• reprogramming
• induced pluripotent stem cells (iPSCs)
• transcription factors
• mouse embryonic fibroblasts (MEFs)
• fast kinetics