Microscopic and biometric investigations of the testes in adult ram and adult buck in Al-Najaf Al-Ashraf Province

Abstract

Microscopic and biometric exploration of the testis in ten of the healthy mature rams and buck choose from AL-Najaf slaughterhouse. Which anticipation to support the future studies and clinical purposes of the biology of the reproductive system. Testicular histological technique civilized then. Microstructures, histodimentions, were recorded then discussed between two target species. Testicular surrounded by a thick sheet of dense irregular connective tissue, tunica albuginea. The last secured by a visceral layer of the tunica vaginalis. The mean thickness in these tunics of an adult ram and buck differed significantly (p≤0.05). The parenchyma was subdivided into lobules by septae extend from mediastinum testis toward tunica albuginea. Each lobule of the two target species contains tortuous seminiferous tubule. Interstitial connective tissue surrounds each tubule contain blood vessels, fibroblasts loose connective tissue cells, and Leydig cells. The mean number, the diameter of Leydig cells and their nuclei in microscopic fields in ram and buck differed significantly (p≤0.05). Sertoli cells pyramidal shaped with ovoid nuclei mean diameter of Sertoli cells in ram and buck differed significantly (P≤0.05). Spermatogonia located near the basal membrane, the Mean diameter of Spermatogonia, in ram and buck differed significantly (p≤0.05). Large primary spermatocytes with largely rounded nuclei with coarse clumps of chromatin mean diameter in two species differed significantly (p≤0.05). Secondary spermatocytes smaller than primary. Small spermatids were rounded. Mature one characterized to elongate. Seminiferous tubule continuous as straight tubules with rete testis tubules lined by simple squamous epithelium. The later end with ductuli efferentes lined with tall ciliated columnar epithelium. Which continued into ductus epididymis lined with ciliated pseudostratified columnar epithelium.