Effect of treadmill training on fibrocartilage complex repair in tendon-bone insertion healing in the postinflammatory stage

Jindong Tan; Xiao Liu; Mei Zhou; Feng Wang; Lin Ma; Hong Tang; Gang He; Xia Kang; Xuting Bian; Kanglai Tang

doi:10.1302/2046-3758.125.BJR-2022-0340.R2

Bone & Joint Research (May 2023)

Effect of treadmill training on fibrocartilage complex repair in tendon-bone insertion healing in the postinflammatory stage

Jindong Tan,
Xiao Liu,
Mei Zhou,
Feng Wang,
Lin Ma,
Hong Tang,
Gang He,
Xia Kang,
Xuting Bian,
Kanglai Tang

Affiliations

Jindong Tan: State Key Laboratory of Trauma, Burn and Combined Injury, Department of Orthopaedics/Sports Medicine Center, First Affiliated Hospital of Army Medical University, Chongqing, China
Xiao Liu: State Key Laboratory of Trauma, Burn and Combined Injury, Department of Orthopaedics/Sports Medicine Center, First Affiliated Hospital of Army Medical University, Chongqing, China
Mei Zhou: State Key Laboratory of Trauma, Burn and Combined Injury, Department of Orthopaedics/Sports Medicine Center, First Affiliated Hospital of Army Medical University, Chongqing, China
Feng Wang: State Key Laboratory of Trauma, Burn and Combined Injury, Department of Orthopaedics/Sports Medicine Center, First Affiliated Hospital of Army Medical University, Chongqing, China
Lin Ma: State Key Laboratory of Trauma, Burn and Combined Injury, Department of Orthopaedics/Sports Medicine Center, First Affiliated Hospital of Army Medical University, Chongqing, China
Hong Tang: State Key Laboratory of Trauma, Burn and Combined Injury, Department of Orthopaedics/Sports Medicine Center, First Affiliated Hospital of Army Medical University, Chongqing, China
Gang He: State Key Laboratory of Trauma, Burn and Combined Injury, Department of Orthopaedics/Sports Medicine Center, First Affiliated Hospital of Army Medical University, Chongqing, China
Xia Kang: State Key Laboratory of Trauma, Burn and Combined Injury, Department of Orthopaedics/Sports Medicine Center, First Affiliated Hospital of Army Medical University, Chongqing, China
Xuting Bian: State Key Laboratory of Trauma, Burn and Combined Injury, Department of Orthopaedics/Sports Medicine Center, First Affiliated Hospital of Army Medical University, Chongqing, China
Kanglai Tang: State Key Laboratory of Trauma, Burn and Combined Injury, Department of Orthopaedics/Sports Medicine Center, First Affiliated Hospital of Army Medical University, Chongqing, China

DOI: https://doi.org/10.1302/2046-3758.125.BJR-2022-0340.R2
Journal volume & issue: Vol. 12, no. 5
pp. 339 – 351

Abstract

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Aims: Mechanical stimulation is a key factor in the development and healing of tendon-bone insertion. Treadmill training is an important rehabilitation treatment. This study aims to investigate the benefits of treadmill training initiated on postoperative day 7 for tendon-bone insertion healing. Methods: A tendon-bone insertion injury healing model was established in 92 C57BL/6 male mice. All mice were divided into control and training groups by random digital table method. The control group mice had full free activity in the cage, and the training group mice started the treadmill training on postoperative day 7. The quality of tendon-bone insertion healing was evaluated by histology, immunohistochemistry, reverse transcription quantitative polymerase chain reaction, Western blotting, micro-CT, micro-MRI, open field tests, and CatWalk gait and biomechanical assessments. Results: Our results showed a significantly higher tendon-bone insertion histomorphological score in the training group, and the messenger RNA and protein expression levels of type II collagen (COL2A1), SOX9, and type X collagen (COL10A1) were significantly elevated. Additionally, tendon-bone insertion resulted in less scar hyperplasia after treadmill training, the bone mineral density (BMD) and bone volume/tissue volume (BV/TV) were significantly improved, and the force required to induce failure became stronger in the training group. Functionally, the motor ability, limb stride length, and stride frequency of mice with tendon-bone insertion injuries were significantly improved in the training group compared with the control group. Conclusion: Treadmill training initiated on postoperative day 7 is beneficial to tendon-bone insertion healing, promoting biomechanical strength and motor function. Our findings are expected to guide clinical rehabilitation training programmes. Cite this article: Bone Joint Res 2023;12(5):339–351.

Published in Bone & Joint Research

ISSN: 2046-3758 (Online)
Publisher: The British Editorial Society of Bone & Joint Surgery
Country of publisher: United Kingdom
LCC subjects: Medicine: Internal medicine: Specialties of internal medicine: Diseases of the musculoskeletal system
Website: https://online.boneandjoint.org.uk/journal/bjr

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