On-Demand Bioadhesive Dendrimers with Reduced Cytotoxicity
Feng Gao,
Ivan Djordjevic,
Oleksandr Pokholenko,
Haobo Zhang,
Junying Zhang,
Terry W. J. Steele
Affiliations
Feng Gao
School of Material Science and Engineering, Beijing University of Chemistry Technology, North Third Ring Road 15, Chaoyang District, Beijing 100029, China
Ivan Djordjevic
Escuela de Ingeniería y Ciencias, Tecnologico de Monterrey, Ave. Eugenio Garza Sada 2501, Monterrey 64849, NL, Mexico
Oleksandr Pokholenko
School of Materials Science and Engineering, Division of Materials Technology, Nanyang Technological University, Singapore 639798, Singapore
Haobo Zhang
School of Material Science and Engineering, Beijing University of Chemistry Technology, North Third Ring Road 15, Chaoyang District, Beijing 100029, China
Junying Zhang
School of Material Science and Engineering, Beijing University of Chemistry Technology, North Third Ring Road 15, Chaoyang District, Beijing 100029, China
Terry W. J. Steele
School of Materials Science and Engineering, Division of Materials Technology, Nanyang Technological University, Singapore 639798, Singapore
Tissue adhesives based on polyamidoamine (PAMAM) dendrimer, grafted with UV-sensitive aryldiazirine (PAMAM-g-diazirine) are promising new candidates for light active adhesion on soft tissues. Diazirine carbene precursors form interfacial and intermolecular covalent crosslinks with tissues after UV light activation that requires no premixing or inclusion of free radical initiators. However, primary amines on the PAMAM dendrimer surface present a potential risk due to their cytotoxic and immunological effects. PAMAM-g-diazirine formulations with cationic pendant amines converted into neutral amide groups were evaluated. In vitro toxicity is reduced by an order of magnitude upon amine capping while retaining bioadhesive properties. The in vivo immunological response to PAMAM-g-diazirine formulations was found to be optimal in comparison to standard poly(lactic-co-glycolic acid) (PLGA) thin films.
