Phenotypic traits and SSR molecular identification of hybrid progenies of Camellia chekiangoleosa × C. semiserrata

Abstract

Camellia chekiangoleosa has high oil concentration and oleic acid content, while C. semiserrata has strong growth vigor and resistance. In order to make the utmost of the advantages of C. chekiangoleosa and C. semiserrata and cultivate excellent germplasm materials, the phenotypic traits of 45 F1 hybrid progenies of C. chekiangoleosa and C. semiserrata were analyzed to grasp the phenotypic traits of the hybrid progenies. In addition, SSR markers were used to identify hybrids, and SSR markers that could be used to identify the hybrid progenies of oil tea were screened. The results were as follows:(1)The F1 hybrids of C. chekiangoleosa × C. semiserrata showed tall tree and rapid growth, and their leaf veins, sepals and stigmas were all tended to the traits of the male parent C. semiserrata, while flower and leaf morphologies and other traits were similar to the female parent C. chekiangoleosa, and the characteristics of leaf color and size were between those of the parents.(2)From the 32 SSR markers, eight fully complementary markers that could distinguish parents and determine the origin of offspring were screened out for identification of hybrid progenies, among which the hybrid identification rates of seven markers were as high as 100%, and the hybrid identification rate of one marker was 55.56%. And 45 hybrid progenies were all true hybrids identified by the complementarity of eight markers.(3)The eight SSR markers were used to verify the ability to identify the hybrid progenies, indicating that it is feasible to use these SSR markers to identify the authenticity of the hybrid progenies of oil tea. This study results provide a reference for interspecies cross breeding of oil tea, and also provide a reference for the SSR marker identification of hybrids of oil tea.

Keywords

• camellia chekiangoleosa
• hybrid progeny identification
• ssr markers
• phenotypic traits